贵州医科大学学报

2009, v.34;No.141(06) 622-624

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海洛因依赖对大鼠垂体β-内啡肽表达的影响
The Effects of Heroin Dependence on Expression of β-endrophin in Pituitary Cells of Rats

梁文妹;李一欣;夏白娟;潘贵书;
LIANG Wenmei1,LI Yixin1,XIA Baijuan1,PAN Guishu2(1.Department of Histology and Embryology,Guiyang Medical College,Guiyang 550004,Guizhou,China;2.Departmeat of Physiology,Zunyi Medical College,Zunyi 563003,Guizhou,China)

摘要(Abstract):

目的:探讨海洛因依赖对大鼠垂体远侧部β-内啡肽(β-endrophin,β-EP)表达的影响。方法:成年雄性SD大鼠55只,随机分为正常对照组、盐水对照组及海洛因依赖组;皮下注射海洛因建立海洛因依赖大鼠模型,分别于模型建立的第10、17、24、31、38天取垂体组织,应用免疫组织化学SABC法、图像分析法观察大鼠垂体远侧部β-内啡肽表达。结果:与正常对照组和盐水对照组比较,海洛因依赖组大鼠垂体远侧部β-EP免疫反应明显减弱,平均灰度值增高(P<0.05)。结论:在海洛因依赖期间,垂体远侧部β-EP表达减少,提示海洛因依赖使内源性阿片样物质的产生受到抑制。
Objective:To investigate the effect of heroin dependence on the expression of β-endrophin(β-EP)in pars distalis hypophyseos for exploring the possible mechanism of the changes.Methods:Fifty-five male adult SD rats were randomly divided into a normal control group(group NCG),a saline control group(group SCG)and a heroin dependence group(group HDG).Heroin-dependent rat models were set up by subcutaneous injection of heroin and the hypophysis were excised on the 10th,17th,24th,31th,and 38th days after models were set up.Immunohistochemical SABC method,imagine analysis,and radioimmunoassay were used in the research.Results:Compared with those of groups NCG,and SCG,the immune staining intensity of β-EP cells in group HGD was weaker,and the average gray value was significantly higher(P<0.05).Conclusions:β-EP positive cells diminish during heroin dependence,The morphological and functional changes suggest that heroin inhibit the production of endogenous opioid.

关键词(KeyWords): 海洛因依赖;β内啡肽;垂体;免疫组织化学;大鼠,Sprague-Dawley
heroin dependence;beta-endorphin;pituitary gland;immunohistochemistry;rats,Sprague-dawley

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基金项目(Foundation): 贵州省优秀科技人才省长基金(2005)14号;; 高层次人才基金(2007)26号

作者(Author): 梁文妹;李一欣;夏白娟;潘贵书;
LIANG Wenmei1,LI Yixin1,XIA Baijuan1,PAN Guishu2(1.Department of Histology and Embryology,Guiyang Medical College,Guiyang 550004,Guizhou,China;2.Departmeat of Physiology,Zunyi Medical College,Zunyi 563003,Guizhou,China)

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DOI: 10.19367/j.cnki.1000-2707.2009.06.009

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