罗天永,吴君,周晓倩
LUO Tianyong,WU Jun,ZHOU Xiaoqian(Department of Infectious Diseases

• 1:贵阳医学院附院感染科
• 2:贵阳医学院附院感染科
• 3:贵阳医学院附院感染科 贵州贵阳550004
• 4:贵州贵阳550004

摘要(Abstract)：

目的:观察银杏制剂在复合病因大鼠肝纤维化的治疗效果。方法:健康雄性Wistar大鼠70只,随机分为正常组、模型组、自然恢复组、治疗组,除正常组外,其余各组用四氯化碳(CCl4)、饮酒、高脂低蛋白饮食等复合因素诱导肝纤维化模型,8周造模结束后治疗组予以银杏制剂灌胃治疗8周,取各组大鼠肝组织HE染色作病理纤维化分级,测定肝脏指数、血清透明质酸(HA)、肝组织羟脯氨酸(Hyp)含量。结果:模型组、自然恢复组大鼠肝纤维化程度、肝脏指数、血清HA和肝组织Hyp较正常组显著升高(P<0.05),治疗组与模型组、自然恢复组相比肝纤维化程度明显改善(P<0.05),肝脏指数、HA、Hyp显著下降(P<0.05)。结论:本实验结果提示银杏制剂对肝纤维化有良好的治疗作用。
Objective: To investigate the therapeutic effect of Gingo biloba preparation in rat hepatic fibrosis induced by combined factors.Methods: Seventy male Wistar rats were divided into normal group,model group,naturally recovering group,and treament group randomly.All of the rats except those in normal group were influenced by combined inducing factors including CCL4,alcohol,and high fat/low protein food for 8 weeks to cause hepatic fibrosis.Then,the rats in treatment group were administered with Gingo biloba via stomach-tube-pouring for 8 weeks.Liver indexes,serum hyaluronic acid(HA),and hydroxyproline(Hyp) contents in liver tissue were measured,and the extent of the liver fibrosis was observed under light microscope.Results: Compared with rats in normal group,the liver indexes,HA and Hyp contents rose significantly(P<0.05) in model group and naturally recovering group,while compared with rats in model group and recovering group,the liver indexes,HA,and Hyp contents of the treatment group fell significantly(P<0.05).Conclusion: Gingo biloba has an anti-fibrogenic effect.

关键词(KeyWords)： 纤维化;肝疾病;银杏
fibrosis;liver diseases;Ginkgo biloba

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 罗天永,吴君,周晓倩
LUO Tianyong,WU Jun,ZHOU Xiaoqian(Department of Infectious Diseases

DOI: 10.19367/j.cnki.1000-2707.2007.03.014

参考文献(References)：

• [1]程明亮,杨长青.肝纤维化的基础研究及临床[M].2版.北京:人民卫生出版社,2002.1.
• [2]刘诗权,于皆平,贺磊,等.核因子k B和转化生长因子B1在银杏叶提取物抗纤维化中的作用[J].中华肝脏病杂志,2005(12):903-907.
• [3]马庭芳,胡伟,于皆平,等.银杏叶提取物对实验性大鼠肝纤维化抑制作用的研究[J].数理医药学杂志,2003(5):396.
• [4]叶昭红,苏文,张介眉,等.银杏叶提取物对大鼠肝纤维化的治疗作用[J].中西医结合肝病杂志,2006(3):147-149.
• [5]孟维利,范连科,李光荣,等.拉米夫定联合银杏叶提取物对慢性乙肝肝纤维化指标的影响[J].临床肝胆病杂志,2005(3):178-179.
• [6]缪杨德,王国祥,宋刘来.银杏叶片治疗失代偿期肝硬化临床疗效观察[J].现代医药卫生,2006(18):2765-2767.
• [7]刘霆,陈玉祥,赵颜忠,等.银杏内酯软胶囊毒理学研究[J].中国现代医学杂志,2003(6):24-27.
• [8]J Gillibert Duplantier,L Dubuisson,N Senant.A role forthrombin in liver fibrosis[J].Gut,2004(53):1682-1687.
• [9]刘杜霞.银杏叶的研究进展[J].国外医药植物药分册,1994(11):10-11.
• [10]刘嫒文.银杏药理作用的研究进展[J].时珍国药研究,1997(6):564-565.
• [11]薛慧,李玉山,谭志鑫.银杏叶茶对高胆固醇饮食小鼠血脂的影响[J].中国现代医学杂志,2001(4):51-52.
• [12]何云,袁凤仪,任渝江,等.银杏内酯对小鼠急性肝坏死的保护作用[J].中华传染病杂志,2001(6):368-370.
• [13]Bruck R,Shirin H,Aeed H.Prevention of hepatic cirrho-sis in rats by hydroxyl radical scavengers[J].J Hepatolo-gy,2001(5):457.

文章评论(Comment)：