曾明辉
Zeng Minghui Department of Anatomy

• 1: 贵阳医学院人体解剖学教研室

摘要(Abstract)：

本文分别用SNP和AHM作TMB反应的稳定剂,对树鼩脊髓前角运动神经元作了比较观察,结果发现两种成色反应对显示神经元胞体和近端树突无明显差别,对显示远端树突,TMB-SNP反应明显优子TMB-AHM反应。同时,本文采用的玻璃纸平板包埋法较Aldes法简便,且效果相同。
The morphology of the spinal anterior horn motoneurons in the tree shrew was comparatively studied under light microscope by using of SNP (sodium nitrop- russide, SNP) and AHM (ammonium heptamolybdate, AHM) as the stablizer in TMB(tetramethyl benzidine, TMB)chromogenic reactions of the CB-HRP method respectively. Then, the sections reacted by TMB-AHM were embedded with the technique of combined flat-embedding of glass paper(the modified Aldes' combi- ned flat-embedding technique)for electron microscopic observation, It was found that: both TMB-SNP and TMB-AHM reactions shrow the features of the neuron bodies and their proximal dendrites similarly; while in the features of the distal dendrites, especially the dendrites in the white matter, TMB-SNP reaction was more excellent than TMB-AHM On the other hand, under electron microscope, the ultrastructures(synapse, neurofibriles, mitochondria, etc. )were clearly seen in the samples of TMB-AHM reaction, but foggily in TMB-SNP reaction. The procedure of glass paper flat-embedding technique is more simple than that of the Aldes' original technique, but its electron microscopic effect is same with that of the original technique.

关键词(KeyWords)： 辣根过氧化物酶类;四甲基联苯胺;硝普钠;庚钼酸铵
horseradish peroxidase; tetramethyl benzidine; sodium nitroprussde ammonium heptamolybdate.

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 曾明辉
Zeng Minghui Department of Anatomy

DOI: 10.19367/j.cnki.1000-2707.1989.02.013

参考文献(References)：

• 1． 鞠 躬,等。神经解剖学方法。第1版。北京:人民卫生出版社,1985:76～121。
• 2． Mesulam M-M, et al. Sensitivity in horseradish peroxidase neurohistochemistry; A comparative study of nine methods. J Histochem Cytochem 1979; 27; 763-773．
• 3． Aldes L D, et al. A combined flat-embedding, HRP histochemical method for correlative light and electron microscopic study of single neurons. J Neurosci Res 1984; 11: 27-34．

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