王梅竹,王颜颜,曹煜,赵亮,李小玲,刘涛华,康颖倩
WANG Meizhu,WANG Yanyan,CAO Yu,ZHAO Liang,LI Xiaoling,LIU Taohua,KANG Yingqian

• 1:贵阳医学院微生物学教研室
• 2:贵阳医学院附院皮肤性病科

摘要(Abstract)：

目的:研究临床不同来源白念珠菌25S rDNA基因型分布特点,探讨白念珠菌不同基因型与感染部位或不同感染类型之间的联系。方法:特异性扩增不同来源的185株白念珠菌25S rDNA基因Ⅰ型内含子序列,再经过琼脂糖凝胶电泳方法对扩增条带进行基因分型,并对不同感染部位、不同感染类型的白色念珠菌的基因型进行比较。结果:185株白念珠菌中,A型122株(65.9%)、B型35株(18.9%)和C型28株(15.1%),未发现D型与E型菌株;外阴阴道念珠菌病(VVC)来源与深部组织来源的白念珠菌25S rDNA基因型差异具有统计学意义(P=0.000);深部组织来源菌株中,艾滋病相关性和非艾滋病相关性白念珠菌基因型差异无统计学意义(P=0.680)。结论:VVC来源和深部组织来源的白念珠菌25S rDNA基因型不同,而深部组织来源的艾滋病相关性和非艾滋病相关性白念珠菌25S rDNA基因型相同。
Objective: To investigate genotype distribution of Candida albicans( C. albicans)25S rDNA,and to probe the relationship between different genotypes and infection types or parts.Methods: Specific amplication of 185 C. albicans 25 S rDNA Type I intron sequences,genotyping were carried out after agarose gel electrophoresis,and statistics comparison was conducted on the genotype of C. albicans on different infectious parts or types. Results: 185 C. albicans were classified into three genotypes: 122 were genotype A( 65. 9%),35 were genotype B( 18. 9%) and 28 were genotype C( 15. 1%),but the genotype D and genotype E were not found. There was statistical significance of the differences of C. albicans 25 S rDNA genotypes between vulvovaginal candidiasis( VVC) sources and invasive infection sources( P = 0. 000),but for the invasive infection sources,there was no significant difference of C. albicans 25 S rDNA genotypes among the HIV /AIDS-related and non HIV /AIDSrelated strains( P = 0. 680). Conclusions: There are different characteristics between VVC sources and invasive infection sources strains by 25 S rDNA genotype,but for the invasive infection sources,the HIV /AIDS-related and non HIV /AIDS-related strains by 25 S rDNA genotypes have the similar distribution characteristics.

关键词(KeyWords)： 念珠菌,白色;基因型;基因扩增;念珠菌,外阴阴道;感染;25S rDNA
Candida albicans;genotypes;gene amplication;candidiasis,vulvovaginal;infection;25S rDNA

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金地区科学基金项目(31060006,31260029);; 贵州省科技厅社会发展科技攻关项目[黔科合SY字(2011)3017号];; 贵州省卫生厅科技项目(gzwkj2010-1-025);; 贵阳市科技局社会发展与民生计划[筑科合同(2011)10316号]

作者(Author): 王梅竹,王颜颜,曹煜,赵亮,李小玲,刘涛华,康颖倩
WANG Meizhu,WANG Yanyan,CAO Yu,ZHAO Liang,LI Xiaoling,LIU Taohua,KANG Yingqian

DOI: 10.19367/j.cnki.1000-2707.2014.05.005

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