贵州医科大学学报

2008, No.134(05) 524

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根癌农杆菌介导灭蚊卵菌贵阳腐霉的遗传转化(英文)
The genetic transformation of Pythium guiyangense mediated by Agrobacterium tumefaciens

赵竟男;苏晓庆;
ZHAO Jingnan, SU Xiaoqing Department of Biology,Guiyang Medical College,Guiyang 550004,China

摘要(Abstract):

Pythium guiyangense is a mosquito pathogen,and has been proved to be a promising agent for biological control of mosquitoes.In order to develop the strains adaptable to different ecological environment having stable virulence to mosquito larvae,and being able to prolong the shelf life,an effort was made on transforming the fungus by using homologous or heterologous virulence genes.In this paper,a genetic transformation experiment of P.guiyangense mediated by Agrobacterium tumefaciens is reported.As a result,an A.tumefaciens mediated genetic transformation system was established successfully.
Pythium guiyangense is a mosquito pathogen,and has been proved to be a promising agent for biological control of mosquitoes.In order to develop the strains adaptable to different ecological environment having stable virulence to mosquito larvae,and being able to prolong the shelf life,an effort was made on transforming the fungus by using homologous or heterologous virulence genes.In this paper,a genetic transformation experiment of P.guiyangense mediated by Agrobacterium tumefaciens is reported.As a result,an A.tumefaciens mediated genetic transformation system was established successfully.

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基金项目(Foundation): 国家自然科学基金项目(30760140)

作者(Author): 赵竟男;苏晓庆;
ZHAO Jingnan, SU Xiaoqing Department of Biology,Guiyang Medical College,Guiyang 550004,China

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DOI: 10.19367/j.cnki.1000-2707.2008.05.030

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