沈艳艳,任婷婷
SHEN Yanyan,REN Tingting

• 1:贵州医科大学医学检验学院临床生物化学教研室

摘要(Abstract)：

目的:探讨蓝莓对大鼠非酒精性脂肪性肝病(NAFLD)的作用及机制。方法:30只SD大鼠随机均分为正常对照组(CG组)、模型组(MG组)及蓝莓干预组(BJ组),CG组给予普通饲料喂养、正常饮食,MG组、BJ组喂饲复合型高脂饲料复制NAFLD实验动物模型,12周时MG组取2只大鼠行肝脏病理切片鉴定造模是否成功; BJ组喂饲造模饲料前7 d给予蓝莓原浆(15 mg/100 g)灌胃,造模成功后,喂饲普通饲料,继续蓝莓干预10 d;于造模后第10天取各组大鼠肝脏及血清标本,观察大鼠肝组织病理学变化,全自动生化分析仪检测大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、甘油三脂(TG)、胆固醇(TC)、高密度脂蛋白胆固醇(HDLc)、低密度脂蛋白胆固醇(LDL-c); ELISA法检测小鼠肝组织丙二醛(MDA)、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)水平。结果:造模后第10天,CG组HE染色肝细胞以中央静脉为中心呈放射状排列,肝索排列整齐; MG组HE染色可见重度脂肪变性,肝小叶结构紊乱,肝细胞胞浆中出现大小不等的脂滴,提示造模成功; BJ组肝细胞排列正常,在中央静脉周围排列呈放射状,比较MG组,脂肪变性明显减轻,接近CG组;与MG组比较,BJ组大鼠血清ALT、AST、TG、TC及LDL-c水平降低,HDL-c水平升高,肝组织中SOD、GSH水平升高,MDA水平降低,差异有统计学意义(P <0. 05)。结论:蓝莓对大鼠NAFLD肝损伤具有明显的保护作用,其机制可能与蓝莓能增加大鼠肝脏抗氧化酶系表达,拮抗脂质诱导的脂代谢紊乱和氧化应激有关。
Objective: To explore the effects and pathogenesis of blueberries on rats with non-alcoholic fatty liver disease(NAFLD). Methods: 30 SD rats were randomly divided into normal control group(CG),NAFLD model group(MG) and blueberry juice group(BJ group). Rats in the CG group were fed with common diet,while rats in the MG group and BJ group were fed with compound high-fat diet to replicate the NAFLD experimental animal model. After 12 weeks,2 rats in MG group were selected to make pathological sections of liver to determine whether the model was successful. The BJ group was treated with blueberry juice(15 mg/100 g) for 7 days before feeding the model feed;After successful modeling,the common feed was fed and the blueberry intervention was continued for 10 days. The liver and serum samples of each group were taken on the 10 th day after the model was made. The pathological changes of liver tissue were observed,and the serum alanine aminotransferase(ALT),aspartate aminotrans-ferase(AST),triglyceride(TG),total cholesterol(TC),low-density lipoprotein-cholesterol(LDL-c) and high-density lipoprotein-cholesterol(HDL-c) levels were detected by bautomatic biochemical analyzer. The superoxide dismutase(SOD) activity,reduced glutathione(GSH) levels and malondialdehyde(MDA) contents levels in liver tissue were quantified by ELISA.Results: On the 10 th day after modeling,the HE stained hepatocytes in CG group were arranged radially with the central vein as the center and the hepatic cord arranged neatly. In the MG group,severe steatosis,hepatic lobular structure disorder and lipid droplets of varying sizes were observed in the cytoplasm of hepatocytes,indicating that the model was successful. The hepatocytes in the BJ group were normal and radial around the central vein. Compared with the MG group,steatosis was significantly alleviated,close to that in CG group. Compared with MG group,the content of serum ALT,AST,TG,TC and LDL-c in the BJ group decreased while HDL-c increased; the activity of SOD and GSH in liver tissue increased,while the content of MDA decreased. The differences were statistically significant(P < 0. 05). Conclusion: Blueberries have obvious protective effects on NAFLD liver injury in rats. The pathogenesis may be related to the increase of antioxidant enzyme expression in rats' livers and lipid metabolic disorders and oxidativestres induced by antagonistic lipid.

关键词(KeyWords)： 蓝莓;非酒精性脂肪性肝病;肝损伤;肝脏功能;脂质代谢;氧化应激;免疫组织化学法
blueberry;non-alcoholic fatty liver disease;liver injury;hepatic function;lipid metabolism;oxidativestres;immunohistochemistry methods

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金地区基金资助项目(81560100)

作者(Author): 沈艳艳,任婷婷
SHEN Yanyan,REN Tingting

DOI: 10.19367/j.cnki.1000-2707.2018.11.002

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