冯海潮,孙朝琴,何芸,吴琼,张然,莫非,罗昭逊
FENG Haichao,SUN Chaoqin,HE Yun,WU Qiong,ZHANG Ran,MO Fei,LUO Zhaoxun

• 1:贵州医科大学
• 2:山东聊城人民医院

摘要(Abstract)：

目的:研究苗药头花蓼对淋巴细胞分泌干扰素γ(IFN-γ)及白介素4(IL-4)的影响。方法:制备C57BL/6小鼠脾淋巴细胞悬液,同刀豆蛋白A(Con A)及不同浓度的头花蓼(终浓度分别为:1、2、4、8、16、32、64、128、256、512 mg/L)于37℃、5%CO_2培养箱共培养72 h,细胞活性检测试剂盒(CCK-8)检测头花蓼对体外T淋巴细胞增殖的影响,酶联免疫吸附实验(ELISA)测定细胞上清中的IFN-γ和IL-4含量,分析不同浓度的头花蓼对ConA活化的脾淋巴细胞增殖和分泌IFN-γ、IL-4的影响。结果:头花蓼浓度>128 mg/L时,明显抑制T淋巴细胞的增殖,抑制其分泌IFN-γ的水平,与空白组比较差异有统计学意义(P<0.05);头花蓼浓度为8~128 mg/L时,Con A活化的脾淋巴细胞分泌IFN-γ和IL-4的水平明显高于空白对照,而T淋巴细胞的增殖无明显变化;头花蓼的浓度低于32 mg/L时,Con A活化的淋巴细胞的IFN-γ分泌水平随头花蓼浓度升高而增加。结论:一定浓度的头花蓼刺激小鼠脾淋巴细胞增殖、IFN-γ和IL-4分泌,参与机体细胞免疫过程。
Objective: To investigate the effect of Polygonum capitatum on the secretion of IFN-γ and IL-4 in mice spleen lymphocytes. Methods: A series of concentrations of Polygonum capitatum( final concentrations were 1,2,4,8,16,32,64,128,256,512 mg / L) in splenic lymphocytes of C57 BL /6mice that were activated by Con A for 72 h at the condition of 37 ℃ and 5% CO2。Following Counting Kit-8 was used to detected lymphocytes proliferation and double antibody sandiwich ELISA was used to detect changes,the effect of Polygonum capitatum with different concentration on proliferation and secretion of IFN-γ and IL-4 was analyzed. Results: The T-cell proliferation and IFN-γ secretion was restrained when the concentration of Polygonum capitatum was higher than 128 mg / L. The level of IFN-γ and IL-4 secreted by mice spleen lymphocytes after Con A activation was higher obviously with Polygonum capitatum at the concentration between 8 to128 mg / L,compared with the blank control group( P< 0. 05). T-cell proliferation had no obvious change. When the concentration of Polygonum capitatum was lower than 32 mg / L,secretion amount of IFN-γ increased as the concentration of Polygonum capitatum become higher. Conclusion: Polygonum capitatum at a certain concentration could participate in cellular immunity that stimulate the mice spleen lymphocytes proliferation,and secretion IFN-γ and IL-4 in vitro.

关键词(KeyWords)： 植物药;淋巴细胞;头花蓼;干扰素γ;白介素4
Polygonum capitatum;spleen lymphocytes;interferon-γ;interleukin-4

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术基金[黔科合(2014)2027号];; 贵州省科技合作计划基金[黔科合LH(2015)7417号];; 贵州省卫生计生委科学技术基金(gzwikj2015-1-003);; 贵阳市科技局计划项目[筑科合同(2014001)]

作者(Author): 冯海潮,孙朝琴,何芸,吴琼,张然,莫非,罗昭逊
FENG Haichao,SUN Chaoqin,HE Yun,WU Qiong,ZHANG Ran,MO Fei,LUO Zhaoxun

DOI: 10.19367/j.cnki.1000-2707.2016.06.006

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