刘彬,刘慰华,张竞之,孙学刚,黄桂琼,周迎春,刘世明
LIU Bin,LIU Weihua,ZHANG Jingzhi,SUN Xuegang,HUANG Guiqiong,ZHOU Yingchun,LIU Shiming

• 1:广州医科大学附属第二医院心血管疾病研究所
• 2:南方医科大学中医药学院

摘要(Abstract)：

目的:研究毛蕊异黄酮对人黑色素瘤细胞A375增殖、迁移及侵袭的影响。方法:以人黑色素瘤细胞A375为研究对象,MTT法检测不同浓度的毛蕊异黄酮对A375细胞活性的影响,划痕实验检测A375细胞的迁移,Transwell小室检测A375细胞的侵袭作用;qPCR检测A375细胞中基质金属蛋白酶2(MMP-2)及基质金属蛋白酶9(MMP-9)表达水平。结果:毛蕊异黄酮在6.25~100μmol/L浓度内不能抑制A375细胞的增殖,划痕实验及Transwell检测结果显示毛蕊异黄酮可显著抑制A375细胞的迁移及侵袭(P<0.01),qPCR结果显示毛蕊异黄酮能抑制A375细胞MMP-2及MMP-9的表达。结论:毛蕊异黄酮在小于100μmol/L浓度时抑制A375细胞的迁移及侵袭,其机制可能与抑制MMP-2及MMP-9表达有关。
Objective: To investigate the effect of calycosin on proliferation,migration,and invasion of human melanoma cell A375. Method: Human melanoma cell A375 was chosen as study subject; The cell viability was determined by MTT assay after treated with calycosin,and the migration and invasion of A375 cells were measured by scratch test and transwell assay; qPCR was used to detect the expression levels of matrix metalloproteinase-2( MMP-2) and matrix metalloproteinase-9( MMP-9) in A375 cells. Result: Calycosin could not inhibit A375 cell proliferation in the concentration range of 6. 25 ~ 100 μmol / L. Calycosin can inhibit A375 cell migration and invasion,and qPCR results confirmed that calycosin could inhibit the mRNA expression of MMP-2 and MMP-9. Conclusion: When the concentration is less than 100 μmol / L,calycosin can attenuate A375 cell migration and invasion, and the mechanism may relate to inhibiting the mRNA expression of MMP-2 and MMP-9.

关键词(KeyWords)： 黑色素瘤;基质金属蛋白酶;毛蕊异黄酮;A375细胞;迁移;侵袭;增殖
melanoma;matrix metalloproteinases;calycosin;A375 cells;migration;invasion;proliferation

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(No.81302892,No.81173459,No.81373575,No.81202841);; 广东省自然科学基金(No.S2013040016226,No.S2013010014777);; 高等学校博士学科点专项科研基金(No.20124433110019);; 中国博士后基金(No.2013M530363);; 广州医科大学博士启动项目(No.2012C50)

作者(Author): 刘彬,刘慰华,张竞之,孙学刚,黄桂琼,周迎春,刘世明
LIU Bin,LIU Weihua,ZHANG Jingzhi,SUN Xuegang,HUANG Guiqiong,ZHOU Yingchun,LIU Shiming

DOI: 10.19367/j.cnki.1000-2707.2014.02.006

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