贵州医科大学学报

2019, v.44;No.229(10) 1156-1160

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

妇科再造丸含药血清对人子宫肌瘤细胞Bax和Bcl-2蛋白表达的影响
Effect of Fukezaizao Pill Medicated Serum on the Expression of Bax and Bcl-2 Proteins in Human Uterine Fibroid Cells

胡颖;罗俊;张晴;叶兰;胡晓燕;郑涛;
HU Ying;LUO Jun;ZHANG Qing;YE Lan;HU Xiaoyan;ZHENG Tao;Department of Pharmacology, School of Basic Medical Science, Guizhou Medical University;Functional Laboratory, School of Basic Medical Science, Guizhou Medical University;The Affiliated Hospital of Guizhou Medical University;The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine;

摘要(Abstract):

目的:探讨妇科再造丸(FKW)含药血清对体外培养人子宫肌瘤细胞凋亡相关蛋白Bax和B细胞淋巴瘤-2(Bcl-2)表达的影响。方法:对人子宫肌瘤细胞进行原代培养、传代并进行鉴定;60只SD雌性大鼠随机均分为空白组(无药血清组),FKW高、中、低剂量组和桂枝茯苓胶囊(GZFL)组,利用各组大鼠制备无药血清和含药血清分别作用于子宫肌瘤细胞72 h时,采用Western Blot检测细胞凋亡相关蛋白Bcl-2、Bax的表达。结果:培养的细胞经鉴定证实为人子宫肌瘤细胞;与无药血清组比较,FKW含药血清各剂量组均能增加Bax蛋白相对表达量,降低Bcl-2蛋白相对表达量(P<0.05或P<0.01),且作用随剂量的增加而有增强的趋势(P<0.05或P<0.01)。结论:FKW含药血清可影响人子宫肌瘤细胞凋亡相关蛋白Bcl-2、Bax的表达,这可能是FKW临床应用时缓解子宫肌瘤症状的机制之一。
Objective: To investigate the effect of Fukezaizao pill(FKW) medicated serum on the expression of Bax and Bcl-2 in human uterine fibroid cells in vitro. Methods: Primary culture, passage and identification of human uterine fibroid cells were carried out. Sixty SD female rats were randomly divided into blank group(drug-free serum group), FKW high, medium and low dose groups and Guizhifulin capsule(GZFL) group. When the drug-free serum and drug-containing serum of each group of rats acted on uterine fibroid cells for 72 h respectively, western-blot was used to detect the expression of apoptosis related protein Bcl-2 and Bax. Results: The cultured cells were identified as human uterine fibroid cells. Compared with the non-drug serum group, the relative expression of Bax protein increased and the relative expression of Bcl-2 protein decreased in each dose group of FKW containing serum(P<0.05 or P<0.01), and the effect was enhanced with the increase of dose(P<0.05 or P<0.01). Conclusion: FKW containing serum can affect the expression of apoptosis related protein Bcl-2 and Bax in human uterine fibroid cells, which may be one of the mechanisms of FKW to relieve the symptoms of uterine fibroid.

关键词(KeyWords): 细胞凋亡;妇科再造丸;子宫肌瘤细胞;凋亡相关蛋白;B细胞淋巴瘤-2蛋白;B淋巴细胞瘤-2相关X蛋白
apoptosis;Fukezaizao pill;uterine fibroid cells;apoptosis-related protein;B-cell lymphoma-2 protein;B-cell lymphoma-2 related X protein

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术基金项目[黔科合J字(2014)2011];; 贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY2013-93)

作者(Author): 胡颖;罗俊;张晴;叶兰;胡晓燕;郑涛;
HU Ying;LUO Jun;ZHANG Qing;YE Lan;HU Xiaoyan;ZHENG Tao;Department of Pharmacology, School of Basic Medical Science, Guizhou Medical University;Functional Laboratory, School of Basic Medical Science, Guizhou Medical University;The Affiliated Hospital of Guizhou Medical University;The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine;

Email:

DOI: 10.19367/j.cnki.1000-2707.2019.10.008

参考文献(References):

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享