贵州医科大学学报

2017, v.42;No.205(10) 1179-1182

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131例早期自然流产绒毛细胞培养及染色体核型分析
Cell Culture and Karyotype analysis of 131 Cases of Early Spontaneous Abortion

朱蕊;曾爱群;杜晶春;
ZHU Rui;ZENG Aiqun;DU Jingchun;Department of Gynaecology and Obstetrics of the First Affiliated Hospital of Guangzhou Medical University;Kingmed Inspection Institute of Guangzhou Medical University;

摘要(Abstract):

目的:分析早期自然流产患者的绒毛细胞染色体异常核型分布。方法:取早期自然流产孕妇绒毛组织,用胶原酶和胰蛋白酶消化后于CO2培养箱中培养7 d,贴壁细胞加秋水仙素处理后用G-显带方法观察染色体核型,分析染色体数目和结构异常情况、性别比例及与孕妇的年龄关系。结果:131例绒毛细胞标本中,129例标本培养成功,其中染色体核型异常标本61例,异常核型中三体占52.4%,其次是单体(18%)和三倍体(16.4%);流产胚胎中女性>男性(P<0.05),但是男性或女性胚胎中正常核型和异常核型比较差异无统计学意义;从35岁以上孕妇获取的标本中染色体核型异常比例显著高于35岁及以下孕妇(P<0.05)。结论:绒毛细胞染色体核型分析可初步判断早期自然流产患者的流产病因,对该孕妇此后的成功妊娠有指导意义。
Objective: To analyze chromosomal abnormalities of chorionic villus cell in patients with early spontaneous abortion. Methods: The chorionic villus tissues of women with early spontaneous abortion were digested by collagenase as well as trypsin and cultured in CO2 incubator for 7 days. The karyotype was observed by Giemsa staining method after adherent cells were treated with colchicines.The chromosome number,structural abnormalities,sex ratio and age of the pregnant women were analyzed. Results: In 131 cases of villus samples,129 specimens were successfully cultured. Among them,there were 61 cases of abnormal karyotypes; trisomy in abnormal karyotype was 52. 4%; the monomer was 18%; the triploid was 16. 4%. The majority of aborted embryos were female,but the sex difference was no statistical significance in normal karyotypes and abnormal karyotypes. The proportion of chromosome abnormalities in the specimens obtained from women over 35 years was significantly higher than that of women under 35 years( P < 0. 05). Conclusion: Chorionic villus karyotype analysis can be used to determine the cause of miscarriage in early spontaneous abortion,which is instructive to the successful pregnancy of the pregnant woman.

关键词(KeyWords): 自然流产;绒毛;染色体核型;Giemsa染色;胚胎;妊娠
spontaneous abortion;chorionic villus;karyotype;Giemsa staining;embryo;gestation

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基金项目(Foundation): 广州市科技计划项目(201607010397)

作者(Author): 朱蕊;曾爱群;杜晶春;
ZHU Rui;ZENG Aiqun;DU Jingchun;Department of Gynaecology and Obstetrics of the First Affiliated Hospital of Guangzhou Medical University;Kingmed Inspection Institute of Guangzhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2017.10.015

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