贵州医科大学学报

2015, v.40;No.181(10) 1080-1082

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男性泌尿生殖道炎症患者加德纳菌检测的意义
The Significance of Detection of Gardnerella vaginalis for Male Patients with Genitourinary Inflammation

程树强;夏曙华;印琳;彭晓枫;
CHENG Shuqiang;XIA Shuhua;YIN Lin;PENG Xiaofeng;Department of Clinical Laboratory,the Affiliated Hospital of Guizhou Medical University;Department of Clinical Laboratory,Guizhou Medical University;

摘要(Abstract):

目的:探究男性泌尿生殖道炎症与加德纳菌(GV)感染关系。方法:用革兰染色法对127例前列腺炎(前列腺炎组)、29例泌尿道感染(泌尿道感染组)、66例其它前列腺疾病患者(其他前列腺疾病组)及33例正常男性(对照组)的前列腺液或尿沉渣标本进行GV初筛,对筛检出的GV阳性标本进行GV特异性荧光抗体染色复查,比较2种方法的GV检出率及不同分组间GV阳性检出率。结果:2种染色方法对同一组样本的GV阳性检出率比较,荧光抗体染色法均低于革兰染色法,在前列腺炎组和其他前列腺疾病组差异有统计学意义(P<0.01);革兰染色GV检出阳性率由高至低依次为泌尿道感染组、前列腺炎组、其他前列腺疾病组、对照组,组间比较,差异有统计意义(P<0.05);荧光抗体染色法GV阳性检出率由高至低依次为泌尿道感染组、前列腺炎组、对照组、其他前列腺疾病组,泌尿道感染组、前列腺炎组和对照组(或其他前列腺组)组间两两比较,差异有统计学意义(P<0.05),其他前列腺疾病组与对照组比较,差异无统计学意义(P>0.05)。结论:革兰染色法检测GV存在一定假阳性,前列腺炎、泌尿道感染可能与GV感染有关。
Objective: To investigate the relationship between Gardnerella vaginalis( GV) and male genitourinary inflammation. Methods: Gram staining was used to detect GV in prostatic fluid or urinary sediment of 127 patients with prostatitis( prostatitis group),29 patients with urinary tract infection( urinary tract infection group),66 patients with other prostate disease( other prostate disease group)and 33 normal male( normal control group). Then specific immunofluorescent antibody technique was used to recheck positive results of gram stain and GV positive rates were compared between four groups by above-mentioned methods. Results: For the samples in the same experimental group,the GV positive rates detected by specific immunofluorescent antibody staining were generally lower than those by gram staining,and the results were statistically significant in prostatitis group and other prostate disease group( P < 0. 01). The positive rates detected by gram staining in urinary tract infection group was highest,followed by in prostatitis group,in other prostate disease group and in normal control group,and the differences between each group were statistically significant( P < 0. 05). The positive rates detected by fluorescent antibody staining in urinary tract infection group was highest,followed by in prostatitis group,in normal control group and in other prostate disease group,and the differences between urinary tract infection group prostatitis group,and normal control group( other prostate disease group)were statistically significant( P < 0. 05) but the differences between normal control group and other prostate disease group were not statistically significant( P > 0. 05). Conclusion: There exists some false positives in detecting GV by gram staining method. Prostatitis and urinary tract infection might be related to GV infection.

关键词(KeyWords): 加德纳菌;炎症;泌尿生殖系统;GV荧光抗体染色;革兰染色
Gardnerella vaginalis;inflammation;urinary and reproductive system;GV immunofluo-rescent antibody staining;gram staining

Abstract:

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作者(Author): 程树强;夏曙华;印琳;彭晓枫;
CHENG Shuqiang;XIA Shuhua;YIN Lin;PENG Xiaofeng;Department of Clinical Laboratory,the Affiliated Hospital of Guizhou Medical University;Department of Clinical Laboratory,Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2015.10.022

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