贵州医科大学学报

2006, (06) 517-519+522

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HPLC-ELSD法测定荭叶心通软胶囊中三七皂苷R_1和人参皂苷Rg_1的含量
The Determination of Notoginseng Saponin R_1 and Ginsenoside Rg_1 in Hongye Xintong Soft Capsule with HPLC-ELSD

郑林;王爱民;兰燕宇;李勇军;何迅;王永林;
ZHENG Lin, WANG Ai-min, LAN Yan-yu, LI Yong-jun, HE Xun, WANG Yong-lin* (Schoolt of Pharmacology, Guiyang Medical College, Guiyang 550004, China)

摘要(Abstract):

目的:建立荭叶心通软胶囊中三七皂苷R1和人参皂苷Rg1含量的测定方法。方法:应用高效液相色谱-蒸发光散射检测(HPLC-ELSD)法,采用C18柱,以乙腈-水(20∶80)为流动相,流速1mL/min,柱温40℃,测定荭叶心通软胶囊中三七皂苷R1和人参皂苷Rg1的含量。结果:三七皂苷R1在0·025~0·40g/L范围内呈现良好的线性关系(r=0·9997),平均回收率为98·62%,RSD为2·1%(n=5);人参皂苷Rg1在0·098~1·56g/L范围内呈现良好的线性关系(r=0·9999),平均回收率为97·68%,RSD为1·9%(n=5)。结论:高效液相色谱-蒸发光散射法简便、快速、重现性好,可用于荭叶心通软胶囊的质量控制。
Objective: To establish a HPLC-ELSD method for the determination of notoginseng saponin R_1 and Ginsenoside Rg_1 in Hongye Xintong Soft Capsule. Methods: A Hypersil-C_ 18 column(150mm×4.6mm,5mm)was used with the mobile phase of acetonitrile-water (20:80), at the flow rate of 1mL/min, and the columm temperature of 40℃. Results: Notoginseng saponin R_1 showed a good linear relationship at the range of 0.025-0.40mg/mL (γ=0.9997). The average recovery rate was 98.62%, with RSD=2.1%(n=5). Ginsenoside Rg_1 showed a good linear relationship at the range of 0.098-1.56mg/mL(γ=0.9999). The average recovery rate was 97.68%, with RSD=1.9%(n=5).Conclusions: The method is simple, accurate and reproducible. It can be used for the quality control of Hongye Xintong Soft Capsule.

关键词(KeyWords): 荭叶心通软胶囊;色谱法,高压液相;三七;人参皂苷
Hongye Xintong soft capsule; chromatography, high pressure liquid; panax notoginseng; panaxosides

Abstract:

Keywords:

基金项目(Foundation): 贵州省中药现代化科技产业资助项目[黔科合中药专字(2003)12号]

作者(Author): 郑林;王爱民;兰燕宇;李勇军;何迅;王永林;
ZHENG Lin, WANG Ai-min, LAN Yan-yu, LI Yong-jun, HE Xun, WANG Yong-lin* (Schoolt of Pharmacology, Guiyang Medical College, Guiyang 550004, China)

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DOI: 10.19367/j.cnki.1000-2707.2006.06.009

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