贵州医科大学学报

2012, v.37;No.159(06) 593-596

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贵州省侗族人群mtDNA 9 bp序列缺失频率及DYS287位点多态性研究
Study on Frequency of 9 bp Sequence Deletion of Mitochondrial DNA and Polymorphism of DYS287 Site in Dong Minority of Guizhou Province

王欢;任凌雁;何燕;张婷;王婵娟;官志忠;单可人;
WANG Huan1,REN Lingyan2,HE Yan2,ZHANG Ting2,WANG Chanjuan2,GUAN Zhizhong2,SHAN Keren2(1.Department of Electron Microscopy,Guiyang Medical College,Guiyang 550004,Guizhou,China;2.The Key Laboratory of Molecular Biology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

摘要(Abstract):

目的:分析贵州省侗族人群线粒体DNA 9 bp序列缺失频率及Y染色体DYS287位点的多态性。方法:用PCR-聚丙烯酰胺凝胶法(PAGE)分析mtDNA 9 bp缺失多态性,DNA序列分析法验证结果;用PCR-琼脂糖凝胶电泳检测法分析DYS287位点多态性。结果:在96例侗族男性个体中,mtDNA 9 bp缺失多态频率为13.54%(13/96),DYS287全部显示为YAP-。结论:获得贵州省侗族人群mtDNA 9 bp序列缺失频率及Y染色体DYS287位点多态数据,为该民族群体遗传分析及起源提供了相关遗传背景资料。
Objective: To analyze the frequency of 9 bp sequence deletion of mitochondrial DNA(mtDNA) and the polymorphism of DYS287 site of Y-chromosome in Dong minority of Guizhou province.Methods: PCR and polyacrylamide gel electrophoresis(PAGE) were used to analyze mtDNA 9bp sequence deletion polymorphism.PCR and agarose gel electrophoresis were used to detect DYS287 polymorphism.DNA sequence analysis was used to verify the results.Results: In 96 unrelated Dong individuals,the frequency of mtDNA 9bp sequence deletion was 13.54%(13/96),and YAP element was absent.Conclusions: The genetic data of mtDNA 9bp sequence deletion frequency and Y-chromosome DYS287 site polymorphism in Dong minority are obtained.

关键词(KeyWords): 侗族;贵州;线粒体DNA;DYS287;遗传多态性
Dong ethnic group;Guizhou;mitochondrial DNA;DYS287;genetic polymorphism

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅基金项目[黔科合J字(2011)2119号];; 贵州省科技厅社会发展攻关项目[黔科合SY字(2012)3135号]

作者(Authors): 王欢;任凌雁;何燕;张婷;王婵娟;官志忠;单可人;
WANG Huan1,REN Lingyan2,HE Yan2,ZHANG Ting2,WANG Chanjuan2,GUAN Zhizhong2,SHAN Keren2(1.Department of Electron Microscopy,Guiyang Medical College,Guiyang 550004,Guizhou,China;2.The Key Laboratory of Molecular Biology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

DOI: 10.19367/j.cnki.1000-2707.2012.06.004

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