武飞,肖婉,徐冉,王建塔,汤磊,梁冰
WU Fei,XIAO Wan,XU Ran,WANG Jianta,TANG Lei,LIANG Bing

• 1:贵州医科大学药理学教研室
• 2:贵州医科大学
• 3:贵州医科大学药学院

摘要(Abstract)：

目的:观察天葵-正丁醇提取物(TK)对免疫抑制小鼠细胞因子的影响。方法:50只清洁级昆明种小鼠,随机均分成TK高、中、低剂量组、正常对照组(对照组)、环磷酰胺(CTX)免疫抑制模型组(模型组);TK高、中、低组小鼠分别灌胃200 mg/kg、100 mg/kg及50 mg/kg TK 1次/d,连续11 d;模型组和对照组给予等量生理盐水灌胃;第9、第10及第11天灌胃后,模型组和TK组腹腔注射CTX 100 mg/(kg·d),对照组注射等量生理盐水;小鼠禁食12 h后于第12天取眼球血,酶联免疫吸附法检测血清中白细胞介素-2(IL-2)、白细胞介素-4(IL-4)、白细胞介素-10(IL-10)及干扰素-γ(IFN-γ)的含量。结果:与对照组相比,模型组小鼠血清IL-2、IL-4、IL-10及IFN-γ含量明显降低(P<0.05);与模型组相比,TK组血清中IL-2、IL-4、干扰素-γ的含量明显升高(P<0.05)。结论:TK提取物能够拮抗CTX诱导的免疫抑制作用。
Objective: To investigate the effect of Semiaquilegia adoxoides extract on cytokines in mice. Methods: The mice immunosuppression model was induced by cyclophosphamide( CTX). The active ingredient of Semiaquilegia adoxoides was extracted with n-butyl alcohol( TK solution for short).50 clean grade Kunming mice were randomly divided into high dose TK group,middle dose TK group,low dose TK group,normal control group and CTX model group. High dose TK group,middle dose TK group and low dose TK group received intragastric administration of 200 mg / kg,100 mg / kg and 50 mg / kg TK,respectively,once a day and for consecutive 11 days. CTX model group and normal control group received intragastric administration of the equal dose of saline. CTX model group and TK groups received intraperitoneal injection of CTX on the 9th,10 thand 11thday of intragastric administration of TK while normal control group received injection of the equal dose of saline. After the fasted for12 hours,mice were killed and blood of eyeball was collected. ELISA was adopted to detect the level of IL-2,IL-4,IL-10 and IFN-γ. Results: Compared with the normal control group,the levels of IL-2,IL-4,IL-10 and IFN-γ in serum of CTX model group were significantly decreased( P< 0. 05). Compared with CTX model group,the levels of IL-2,IL-4 and IFN-γ in serum of TK groups were significantly increased( P < 0. 05). Conclusion: Semiaquilegia adoxoides extract can significantly inhibit immunosuppressive action induced by CTX.

关键词(KeyWords)： 天葵;环磷酰胺;免疫抑制;细胞因子;模型,动物
Semiaquilegia adoxoides;cyclophosphamide;immunosuppression cytokines;models,animal

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(No:21102025);; 贵州省中药现代化攻关项目[黔科合ZY(2011)3006]

作者(Author): 武飞,肖婉,徐冉,王建塔,汤磊,梁冰
WU Fei,XIAO Wan,XU Ran,WANG Jianta,TANG Lei,LIANG Bing

DOI: 10.19367/j.cnki.1000-2707.2015.07.004

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