叶川,邓展生,申定珠,张皓,宁旭,李江伟,杨华
YE Chuan1,DENG Zhansheng1,SHEN Dingzhu2,ZHANG Hao3,NING Xu3,LI Jiangwei,YANG Hua3(1.Department of Orthopaedics

• 1:中南大学湘雅医院骨科
• 2:中南大学湘雅医院骨科
• 3:中南大学湘雅医院中西医结合研究所
• 4:贵阳医学院附院骨科

摘要(Abstract)：

目的:研究外消旋聚丙交酯(PDLLA)作为半月板组织工程支架材料应用的可行性。方法:采用胶原酶阶段消化法获取人胚半月板纤维软骨细胞,碱性成纤维细胞生长因子(bFGF)作用于第3代细胞,并用MTT比色法得出最佳效应浓度,扩增后的细胞种植于多聚赖氨酸修饰前、后的PDLLA上,在光镜、荧光显微镜、扫描电镜下观察PDLLA的亲水性、细胞吸附性和细胞增殖情况。结果:bFGF扩增人胚半月板细胞的最佳效应浓度为50μg/L(P<0.05);PDLLA亲水性及细胞吸附性欠佳,予多聚赖氨酸修饰后明显改善,细胞在PDLLA上生长良好,3周时已有大量细胞生长。结论:50μg/L的bFGF可用于体外大量扩增人胚半月板细胞;PDLLA经多聚赖氨酸修饰后可表现出良好的亲水性、细胞吸附性和体外生物相容性,可作为半月板组织工程细胞培养支架材料用。
Objective:To investigate the possibility of PDLLA as fibrocartilage cell scaffold in meniscus tissue engineering.Methods:Human fetal meniscal fibrochond-rocytes were digested with collagenase,the 3rd generation of cells were affected with basic fibroblast growth factor(bFGF),and the dose-response was studied with MTT colorimetric method.The cells cultured onto PDLLA modified by poly-lysine or non-modified were observed with light micros-cope,fluorescence microscope,and scanning electronic microscopy.Results:Fibrocartilage cells affected by bFGF at the concentration of 50μg/L showed statistically higher proliferating rates than those in control group(P<0.05).PDLLA showed good hydrophilia and cell adsorptivity after modified by poly-lysine.Big amount of fibrocartilage cells proliferated onto PDLLA in three weeks.Conclusions:bFGF can promote proliferation of human fetal meniscal cells.PDLLA has good hydrophilia,and adsorptivity,and is biologically compatible with fibrocartilage cells.So,it can be used as scaffold for meniscus tissue engineering.

关键词(KeyWords)： 半月板,胫骨;软骨细胞;组织工程;胚胎
menisci,tibial;chondrocytes;tissue engineering;embryo

Abstract:

Keywords:

基金项目(Foundation): 贵州省卫生厅资助项目(G2005-16)

作者(Author): 叶川,邓展生,申定珠,张皓,宁旭,李江伟,杨华
YE Chuan1,DENG Zhansheng1,SHEN Dingzhu2,ZHANG Hao3,NING Xu3,LI Jiangwei,YANG Hua3(1.Department of Orthopaedics

DOI: 10.19367/j.cnki.1000-2707.2007.06.011

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