梁冰,张爱华,奚绪光,黄晓欣,董学新
LIANG bing1,ZHANG Aihua1,XI Xuguang2,HUANG Xiaoxin3,DONG Xuexin3(1.Department of Toxicology

• 1:贵阳医学院卫生毒理学教研室
• 2:Gnotoxicologie et Cycle Cellulaire
• 3:CNRS
• 4:中国人民解放军第44医院

摘要(Abstract)：

目的:探讨燃煤污染型砷中毒患者谷胱甘肽硫转移酶(GST)基因多态性与GST酶活力的关系,为深入了解砷中毒的致病机制提供实验依据。方法:选择130例燃煤污染型砷中毒患者和140例健康人作为研究对象,采用多重等位基因特异聚合酶链反应(多重PCR)检测GSTM1、GSTT1基因缺失;采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测GSTP1 Ile105Val、GSTO1 Ala140Asp、GSTO2 Asn142Asp基因多态性;采用化学比色法检测谷胱甘肽硫转移酶(GST)活力,并分析上述基因多态性与GST酶活力的关系。结果:GSTT1基因缺失基因型、非缺失基因型以及GSTO2 Asn142Asp基因Asn/Asn、Asn/Asp、Asp/Asp基因型,在砷中毒组和对照组的分布差异有统计学意义(P<0.05);砷中毒患者GST酶活力增高,与对照组相比差异有统计学意义(P<0.05);砷中毒组和对照组GSTO1Ala140Asp基因不同基因型间的GST酶活力有差异(砷中毒组P>0.05,对照组P<0.05);未发现GSTM1、GSTT1、GSTP1 Ile105Val、GSTO2 Asn142Asp多态性与GST酶活力有关。结论:GS-TO1 Ala140Asp基因多态性可能影响燃煤污染型砷中毒患者的GST酶活力,其原因尚需进一步研究。
Objective:To explore the relationship between glutathione S-transferase (GST)genetic polymorphisms and GST activity in coal-buring caused arsenism and provide a scientific basis for a more in-depth explanation to arsenism mechanism. Methods:One hundred ant thirty arsenism patients and 140 healthy people were chosen as subjects of the study. Multiple polymerase chain reaction (multiplex PCR) was used to detect deletion of GSTM1,and GSTT1. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to detect polymorphisms of GSTP1,Ile105Val,GSTO1 Ala140Asp,and GSTO2 Asn142Asp. Chemistry chromatometry method was used to detect GST activity. The relationship between the genetic polymorphisms and GST activity was analyzed. Results:The frequencies of GSTT1(-/-) ,GSTT1(+/+)or(+/-) and genotypes Asn/Asn,Asn/Asp and Asp/Asp of gene GSTO2 Asn142Asp were significantly different between arsenism group and control group(P<0.05). GST activity of arsenism patients increased when compared with that of controls. The difference was statistically significant(P<0.05). GST activity of different genotypes of GSTO1 Ala140Asp was different between arsenism group and control group. Conclusion:Gene polymorphism of GSTO1 Ala140Asp may relate to GST activity of arsenism patients caused by coral-burning. Further research on function and transcription regulation of the gene is needed.

关键词(KeyWords)： 砷中毒;GST基因多态性;谷胱甘肽转移酶
arsenic poisoning; glutathione S-transferase genetic polymorphisms; glutathione S-transferase

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30460123);; 贵州省国际科技合作重点项目基金(黔科合外G字400115号);; 贵州省重大专项基金(黔科合重大专项字6016号);; 贵州省省长基金资助项目[黔省专合字(2008)57号];; 贵州省科技厅基金资助项目[黔科合丁字(2008)2276号]

作者(Author): 梁冰,张爱华,奚绪光,黄晓欣,董学新
LIANG bing1,ZHANG Aihua1,XI Xuguang2,HUANG Xiaoxin3,DONG Xuexin3(1.Department of Toxicology

DOI: 10.19367/j.cnki.1000-2707.2008.06.007

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