贵州医科大学学报

2006, (03) 238-240

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非创伤性双下肢缺血后处理对大鼠I/R心肌梗死面积和PKCα表达的影响
Effects of Non-wounded Leg Ischemic Postconditioning on Sizes of Myocardial Infarction Induced by Ischemia/reperfusion and PKCα Expression in Rats

袁志柳;王艺明;沈正;陈保林;黄达枚;刘兴德;
YUAN Zhi-liu~1,WANG Yi-ming~2,SHEN Zheng,CHEN Bao-lin~3,HUANG Da-mei,LIU Xing-de(1.Department of Cardiac Internal Medicine,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China; 2.Psychology Department,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China; 3.Department of Cardiology,Guizhou Provincial People,s Hospital,Guiyang 550002,China)

摘要(Abstract):

目的:探讨在体条件下,非创伤性双下肢缺血后处理(N-W IPTC)对缺血-再灌注(I/R)心肌梗死面积(IS)和心肌组织中蛋白激酶Cα(PKCα)表达的影响。方法:采用SD大鼠心肌I/R模型,应用Evan’s蓝、TTC染色,观察N-W IPTC对IS的影响;运用免疫组化技术观察N-W IPTC对心肌组织PKCα表达的影响。结果:与I/R组比较,N-W IPTC组的IS显著降低(P<0.01或P<0.05);与I/R组比较,N-W IPTC组心肌细胞膜PKCα表达明显多于I/R组(P<0.01)。结论:N-W IPTC能有效降低I/R心肌的IS,其机制可能与促进心肌细胞膜PKCα的表达有关。
Objective: To observe the protective effects of non-wounded leg ischemic postconditioning (N-WIPTC) on ischemia/reperfusion(I/R) myocardial injury in rats.Methods: 24 Sprague-Dawley rats were divided randomly into four groups and the models of ischemia/reperfusion myocardial injury were produced.The infarction sizes(IS) were measured with Evan's and TTC staining,and the expression of protein kinase C(PKCα) in myocardial tissue was investigated by immunohistochemistry.Results: Compared with those of I/R group,the ISs of N-WIPTC were lower(P<0.01or P<0.05),and the expression of PKCα was significantly higher in N-WIPTC groups(P<0.01).Conclusions: N-WIPTC could significantly decrease the IS during I/R.The mechanism might be the promoted expression of PKCα in myocardial tissue after N-WIPTC.

关键词(KeyWords): 心肌再灌注损伤;蛋白激酶C;心肌梗塞;大鼠
myocardial reperfusion injury;protein kinase C;myocardial infraction;rats

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作者(Author): 袁志柳;王艺明;沈正;陈保林;黄达枚;刘兴德;
YUAN Zhi-liu~1,WANG Yi-ming~2,SHEN Zheng,CHEN Bao-lin~3,HUANG Da-mei,LIU Xing-de(1.Department of Cardiac Internal Medicine,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China; 2.Psychology Department,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China; 3.Department of Cardiology,Guizhou Provincial People,s Hospital,Guiyang 550002,China)

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DOI: 10.19367/j.cnki.1000-2707.2006.03.013

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