吴增波,秘双燕,晏燕
WU Zengbo,MI Shuangyan,YAN Yan

• 1:川北医学院附属医院
• 2:珠海拜博口腔医院

摘要(Abstract)：

目的:探讨肿瘤坏死因子-α(TNF-α)在口腔鳞癌组织中的表达及临床意义。方法:取78例口腔鳞癌手术患者的癌组织与癌旁组织(距离癌组织≥3 cm)标本,采用免疫组织化学法测定TNF-α表达,收集患者性别、年龄、肿瘤直径、病理类型、分化程度、分期及淋巴结转移等临床病理资料、分析不同临床病理状态下TNF-α的表达差异,采用Logistic分析TNF-α表达的影响因素。结果:癌旁组织中TNF-α阳性细胞表达较少或无表达,癌组织中TNF-α阳性表达于细胞的胞质、包膜亦有少量表达、细胞核内无表达;癌组织TNF-α阳性表达率57. 69%(45/78)高于癌旁组织12. 82%(10/78),差异有统计学意义(χ2=6. 942,P=0. 025);癌组织中TNF-α的阳性表达率与肿瘤直径、分化程度、分期及淋巴结转移差异有统计学意义(P <0. 05);多因素Logistic分析结果表明,肿瘤直径、分化程度、分期及淋巴结转移是口腔鳞癌组织TNF-α阳性表达的影响因素(P <0. 05)。结论:口腔鳞癌组织中TNF-α呈高表达,可反映患者疾病严重程度,有望成为口腔鳞癌治疗新靶点。
Objective: To investigate the expression and clinical significance of tumor necrosis factor-α( TNF-α) in oral squamous cell carcinoma. Methods: 78 patients with oral squamous cell carcinoma and adjacent tissues( ≥3 cm from the lesion) were used as specimens. Immunohistochemical method was used to determine the positive expression of TNF-α in different tissues. Clinical information( gender,age,tumor diameter and pathology type,degree of differentiation,tumor stage and lymph node metastasis and other clinicopathological conditions) was collected. Then, analyzing the expression differences of TNF-α under different clinicopathological conditions. Logistic analysis was used to analyze the influencing factors of TNF-α expression. Results: TNF-α-positive cells showed less or no expression in adjacent tissues; TNF-α was positively expressed in the cytoplasm of the cancer tissues,a few expression in capsule,and there was no expression in the nucleus. The positive expression rate of TNF-α in cancer tissue was 57. 69%( 45/78),which was higher than that in adjacent tissue( 12. 82%,10/78),the difference was statistically significant( χ2= 6. 942,P =0. 025); The differences between positive expression rate of TNF-α in cancer tissue and tumor diameter,degree of differentiation, tumor stage, and lymph node metastasis were statistically significant( P < 0. 05). The results of multivariate Logistic analysis showed that tumor diameter,differentiation degree,tumor stage,and lymph node metastasis were the influential factors of positive expression of TNF-α in oral squamous cell carcinoma( P < 0. 05). Conclusion: High expression of TNF-α in oral squamous cell carcinoma tissues can reflect the severity of patients' disease,and it is expected to become a new target for the treatment of oral squamous cell carcinoma.

关键词(KeyWords)： 肿瘤坏死因子-α;口腔鳞癌组织;癌旁组织;动态监测;检验值;多因素分析;Logistic回归分析
tumor necrosis factor-α(TNF-α);oral squamous cell carcinoma;paracancerous tissue;dynamic monitoring;test value;multivariate analysis;Logistic regression analysis

Abstract:

Keywords:

基金项目(Foundation): 南充市市校战略合作项目(18SXHZ0483)

作者(Author): 吴增波,秘双燕,晏燕
WU Zengbo,MI Shuangyan,YAN Yan

DOI: 10.19367/j.cnki.2096-8388.2020.09.011

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