徐春丽,方登驻,杨继红,王培民
XU Chun li;FANG Deng zhu ;YANG Ji hong;WANG Pei-min (Department of Pharmacy;

• 1:贵阳医学院附院药剂科!贵州贵阳550004
• 2:贵阳医学院药学系!99界毕业生
• 3:贵阳医学院附院药剂科!贵州贵阳550004
• 4:贵阳医学院附院药剂科!贵州贵阳550004

摘要(Abstract)：

目的 :建立微量血浆中卡马西平浓度的测定方法。方法 :采用反相高效液相色谱 (HPLC)法 ,以艾司唑仑为内标 ,测定微量血浆中卡马西平浓度。色谱柱 :ShimadzuShimpackCLC -ODS 6 0mm× 15 0mm ,加保护柱 ,流动相为甲醇和水 (6 0∶40 ) ,流速 0 8ml/min ,检测波长为 2 5 4nm。结果 :卡马西平在 2～ 16mg/L浓度范围内线性良好 ,r=0 9996。最低检测限为 4 92 μg/L。卡马西平高、中、低 3种浓度的平均回收率分别为 97 0 0 % ,96 0 0 % ,93 98% ;RSD分别为 1 1% ,3 0 % ,4 1% (n =9)。日内RSD分别为 1 9% ,1 6 % ,6 3% (n =5 )。日间RSD分别为 1 8% ,2 4% ,2 9% (n =5 )。结论 :该方法准确、快速、简便 ,可作为卡马西平血药浓度常规监测方法
Objective: To establish a method for rapid determination of Carbamazepine in human plasma Methods: Using RP-HPLC with Estazolam as internal standard to determine concentration of Carbamazepine in human plasma The separation was performed on shimadzu shimpack CLC-C 18 column with methanol water (60∶40) as mobile phase The flow rate was 0 8 ml/min The wavelength for detection was 254 nm Results: The linear range of Carbamazepine was between 2～16 mg/L with r= 0 999 6 The lowest limit of detection was 4 92 μg/L. The average recoveries for carbamazepine in three different concentrations were 97 00%,96 00%,93 98% with RSD of 1 1%, 3 0% and 4 1% respectively (n=9) The RSD values of intraday and interday were 1 9%,1 6%,6 3% (n= 5) and 1 8%, 2 4%, 2 9% ( n= 5 ) Conclusion: This method is accurate, rapid and easy It can be used as a routine method in TDM

关键词(KeyWords)： 卡马西平;色谱法,高压液相;药代动力学;血药浓度
carbamazepine;chromatography, high pressure liguid;pharmacokinetics; drug plasma concentration

Abstract:

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基金项目(Foundation):

作者(Authors): 徐春丽,方登驻,杨继红,王培民
XU Chun li;FANG Deng zhu ;YANG Ji hong;WANG Pei-min (Department of Pharmacy;

DOI: 10.19367/j.cnki.1000-2707.2001.01.012

参考文献(References)：

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• [2 ]李丙阳 血清中卡马西平的薄层荧光扫描定向测定[J] 药学学报 ,1986 ,2 (18) :6 33.
• [3]黄少平 ,和祖光 ,梁颖彬 测定卡马西平唾液浓度的意义 [J] 实用儿科临床杂志 ,1992 ,7(1) :12 - 14.
• [4 ]孙琛主编 临床用药大全 [M ] 上海 :中国大百科全书出版社 ,1995 6 7.

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