胡晓燕,周从容
HU Xiaoyan,ZHOU Congrong(Department of Obstetrics and Gynecology

• 1:贵阳医学院附院妇产科
• 2:贵阳医学院附院妇产科 贵州贵阳550004
• 3:贵州贵阳550004

摘要(Abstract)：

目的:探讨卵泡液和血清中瘦素(Leptin)与胰岛素样生长因子-I(IGF-I)水平与卵泡发育的关系。方法:应用放射免疫法(R IA)检测67例患者取卵日血清及卵泡液中的Leptin与IGF-I水平。结果:(1)取卵日血清中的Leptin水平为(13.20±5.43)mg/L,显著高于卵泡液中Leptin水平(10.86±5.73)mg/L,P<0.05;卵泡液中的Leptin水平与血清中的Leptin水平呈正相关(r=0.84,P<0.001)。血清中的IGF-I水平为(7.18±4.58)mg/L,显著高于卵泡液中IGF-I水平(4.36±3.03)mg/L(P<0.05),卵泡液中的IGF-I水平与血清中的IGF-I水平呈正相关(r=0.79,P<0.001);(2)卵巢低反应型者的血清及卵泡液中Leptin水平显著高于卵巢中高反应型者,差异有显著性(P<0.05);(3)三种卵巢反应类型的血清及卵泡液中IGF-I水平比较,差异无显著性(P>0.05);(4)卵泡液及血清中的Leptin水平与IGF-I水平无明显的相关性(r=-0.079,P>0.05)及(r=-0.127,P>0.05)。结论:(1)卵泡液中的Leptin及IGF-I均来自血清;(2)高浓度的Leptin可能抑制卵泡发育,而低浓度的Leptin可能对卵泡发育没有影响;(3)体内IGF-I可能与其受体结合后发挥对卵泡发育的调节作用;(4)Leptin与IGF-I之间无明显相关关系。
Objective: To investigate the relationship of the levels of insulin-like growth factor I(IGF-I) and leptin with the development of ovarian follicles.Methods: Radioimmunoassay was used to determine levels of IGF-I and leptin in serum and follicular fluid obtained during oocyte picking-up(OPU) in 67 cases of in vitro fertilization and embryo transfer(IVF-Et).Results:(1) Leptin levels in serum were significantly higher than those in matched follicular fluid,[(13.20±5.43)ng/ml and(10.86±5.73)ng/ml respectively(P<0.05)],and were positively correlated with those in follicular fluid(r=0.84,P<0.001).IGF-I levels in serum were significantly higher than those in matched follicular fluid [(7.18±4.58)ng/ml and(4.36±3.03) ng/ml,respectively,(P<0.05)],and were positively correlated with those in follicular fluid(r=0.84 P<0.001).(2) Leptin levels in serum and follicular fluid of patients with lower ovarian reaction were significantly higher than those of patients with middle and higher ovarian reaction(P﹤0.05).(3) There was no significant difference of IGF-I levels in serum and follicular fluid among patients with different ovarian reaction(P>0.05).(4) There was no significant correlation between leptin and IGF-I levels in serum and follicular fluid(r=-0.079 P>0.05)及(r=-0.127 P>0.05).Conclusions:(1) IGF-I and leptin in follicular fluid come from serum;(2) Higher concentrations of leptin may restrain follicular development,while lower concentrations of leptin may not influence follicular development;(3) The regulation of IGF-I to follicular development is possibly educed after the combination of IGF-I with its receptor;(4) There is no significant correlation between leptin and IGF-I.

关键词(KeyWords)： 超排卵;瘦素;胰岛素样生长因子-I;卵泡液
superovulation;leptin;insulin-like growth factor I;follicular fluid

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 胡晓燕,周从容
HU Xiaoyan,ZHOU Congrong(Department of Obstetrics and Gynecology

DOI: 10.19367/j.cnki.1000-2707.2007.02.016

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