秦川霞,黎中恒,黄磊,饶青,宋晶睿,黄裕兵,李艳梅
QIN Chuanxia,LI Zhongheng,HUANG Lei,RAO Qing,SONG Jingrui,HUANG Yubing,LI Yanmei

• 1:省部共建药用植物功效与利用国家重点实验室&贵州医科大学基础医学院免疫教研室
• 2:贵阳市公共卫生救治中心感染二科

摘要(Abstract)：

目的 探讨泌淋清胶囊(MLQ)对不同白血病细胞株增殖及凋亡的影响。方法 取适量MLQ,采用醇提旋蒸冻干方法配制成100 g/L MLQ母液备用；取对数生长期人红白血病细胞株HEL分为125、250及500 mg/L MLQ组及对照组，取对数生长期人慢性髓系白血病细胞K562分为62.5、125.0、250.0及500.0 mg/L MLQ组及对照组，取对数生长期的人急性淋巴细胞白血病细胞Jurkat分为62.5、125.0及250.0 mg/L MLQ组及对照组，3种细胞均培养不同时间及多个时间点、采用噻唑蓝比色法(MTT)测定各组细胞的存活率；取对数生长期Jurkat细胞，设置150、300及600 mg/L及对照组(600 mg/L浓度等量的溶剂),分别培养24和48 h,采用流式细胞仪检测Jurkat细胞24和48 h的细胞凋亡率。结果 各浓度MLQ组于48 h时，细胞株HEL、K562的存活率均随MLQ浓度的增加而降低(P<0.000 1),各浓度MLQ组Jurkat细胞的存活率随MLQ浓度的增加而降低、细胞活力随时间的延长而下降(P<0.005或P<0.001或P<0.000 1);各浓度MLQ组Jurkat细胞的凋亡率随着MLQ浓度的增加而增高(P<0.000 1)。结论 MLQ可抑制不同类型白血病细胞(HEL、K562及Jurkat)增殖，促进Jurkat细胞凋亡。
Objective To investigate the effect of MLQ on proliferation and apoptosis of different leukemia cell lines. Methods Appropriate amount of MLQ were taken and 100 g/L MLQ liquid was prepared by alcohol extraction and lyophilization. Human erythroleukemia cell line HEL at logarithmic growth stage was divided into 125,250, and 500 mg/L MLQ groups and the control group. The log-growing human chronic myeloid leukemia cell K562 were divided into 62.5, 125.0, 250.0, and 500.0 mg/L MLQ group and control group. The log-growing human acute lymphoblastic leukemia cell Jurkat was divided into 62.5, 125.0, and 250.0 mg/L MLQ group and the control group. All three cells were cultured for different times and at multiple time points, and cell viability of each group was determined by thiazole blue color measurement(MTT). For log growth phase Jurkat cells, 150, 300, and 600 mg/L and control group(600 mg/L equivalent solvent) were cultured for 24 h and 48 h to test the apoptosis rate of Jurkat cells for 24 h and 48 h by the flow cytometry instrument. Results At 48 h, the survival rate of HEL and K562 decreased with increasing MLQ concentration in each concentration MLQ group(P<0.000 1). The viability of Jurkat cells in each concentration of MLQ group decreased with increasing MLQ concentration and cell viability decreased with time(P<0.005 or P<0.001 or P<0.000 1).The rate of apoptosis of Jurkat cells in each MLQ group increased with the increasing MLQ concentration(P<0.000 1). Conclusion MLQ can inhibit the proliferation of different types of leukemia cells(HEL, K562, Jurkat) and promote the apoptosis of Jurkat cells.

关键词(KeyWords)： 白血病;Jurkat细胞;K562细胞;增殖;凋亡;泌淋清胶囊;HEL细胞
leukemia;Jurkat cells;K562 cells;cell proliferation;apoptosis;milinqing capsule;HEL cells

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81872772);; 贵州省自然科学基金项目(黔科合平台人才[2020]5008,黔科合支撑[2020]4Y203,黔科合基础-ZK[2022]一般293,黔科合基础-ZK[2021]一般569)

作者(Author): 秦川霞,黎中恒,黄磊,饶青,宋晶睿,黄裕兵,李艳梅
QIN Chuanxia,LI Zhongheng,HUANG Lei,RAO Qing,SONG Jingrui,HUANG Yubing,LI Yanmei

DOI: 10.19367/j.cnki.2096-8388.2023.06.003

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