王小娜,张禹,王培智,周紫云,高雪,杨勤兴,刘舒媛,李传印,史荔,姚宇峰
WANG Xiaona,ZHANG Yu,WANG Peizhi,ZHOU Ziyun,GAO Xue,YANG Qinxing,LIU Shuyuan,LI Chuanyin,SHI Li,YAO Yufeng

• 1:中国医学科学院医学生物学研究所

摘要(Abstract)：

目的:探讨CD40基因多态性与云南汉族人群肺癌发生发展的相关性。方法:选取云南地区汉族人群肺癌Ⅰ+Ⅱ期患者151例、Ⅲ+Ⅳ期患者316例,同时选择云南地区汉族健康体检人群384例(对照组),采用Taq Man探针基因分型方法对两组被检者CD40基因中rs1883832(C>T)和rs4810485(G>T)2个多态性位点进行基因分型,观察等位基因、基因型及所构建的单倍型在肺癌Ⅰ+Ⅱ期患者、肺癌Ⅲ+Ⅳ期患者和对照组的分布频率。结果:rs4810485位点等位基因G和T在Ⅲ+Ⅳ期肺癌和对照组被检者的分布频率差异具有统计学意义(P=0.013);遗传模式的分析结果经年龄校正后,rs1883832位点在Dominant模式下CC基因型和CT~TT基因型在Ⅰ+Ⅱ期及Ⅲ+Ⅳ期肺癌患者分别与对照组的分布频率比较,差异具有统计学意义(P=0.011和0.008);rs4810485位点在Dominant模式下基因型GG和基因型GT~TTde分布频率比较亦具有统计学意义(P=0.008和0.001)。结论:CD40基因中多态性位点rs4810485位点等位基因G和基因型GG可能是肺癌发生的风险性因素,而rs1883832位点基因型CC可能是肺癌发生的风险因素。
Objective: To investigate the correlation of the single nucleotide polymorphisms( SNPs)( rs1883832 C > T and rs4810485 G > T) in CD40 gene with the occurrence and development of lung cancer in a Yunnan Han population. Methods: A total of 155 lung cancer patients in pathological stages Ⅰ + Ⅱ,316 lung cancer patients in pathological stages Ⅲ + Ⅳ and 384 healthy individuals were recruited in this study. The genotypes of 2 polymorphism loci( rs1883832 C > T and rs4810485 G > T)of CD40 gene were analyzed by Taqman assay. The distribution frequencies of allele,genotype,and haplotypes in lung cancer patients of pathological stages of Ⅰ + Ⅱ and Ⅲ + Ⅳ,and normal control were observed. Results: The allelic frequencies of G and T at locus rs4810485 in group Ⅲ + Ⅳ were significantly different from those in control group( P = 0. 013),suggesting G at the locus of the allele was the risk factor( OR = 1. 33,95% CI: 1. 06 ~ 1. 66). The analysis results of inheritance mode showed that under Dominant mode( best fit mode),at locus rs1883832,there were significant differences of frequencies of genotypes CC and CT ~ TT between Ⅰ + Ⅱ and control,and between Ⅲ + Ⅳand control group( P = 0. 011 and 0. 008). In regard of locus rs4810485,under Dominant mode,the distribution frequncies of genotypes of GG and GT ~ TTde were significantly different between Ⅰ + Ⅱand control,and between Ⅲ + Ⅳ and control group( P = 0. 008 和 0. 001). Conclusions: At polymorphism locus rs4810485 of gene CD40,allele G( OR = 1. 33,95% CI: 1. 06 ~ 1. 66) and genotype GG( OR = 1. 79,95% CI: 1. 17 ~ 2. 74) might be the risk factors of occurrence of lung cancer. While at locus rs1883832,genotype CC be the risk factor of lung cancer initiation( OR = 1. 73,95% CI: 1. 13 ~2. 63).

关键词(KeyWords)： 基因型;肺肿瘤;相关性;云南;CD40
genotype;lung neoplasm;correlation;Yunnan;CD40

Abstract:

Keywords:

基金项目(Foundation): 云南省医学学科带头人培养计划(D-201669);; 云南省科技厅-昆明医科大学应用基础研究联合专项(2013FB064);; 云南省肿瘤内设机构(2014NS139)

作者(Author): 王小娜,张禹,王培智,周紫云,高雪,杨勤兴,刘舒媛,李传印,史荔,姚宇峰
WANG Xiaona,ZHANG Yu,WANG Peizhi,ZHOU Ziyun,GAO Xue,YANG Qinxing,LIU Shuyuan,LI Chuanyin,SHI Li,YAO Yufeng

DOI: 10.19367/j.cnki.1000-2707.2017.04.003

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