贵州医科大学学报

2019, v.44;No.229(10) 1161-1166

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低强度超声辐照对人宫颈癌HeLa细胞凋亡及Casepase-12蛋白表达的影响
Effect of Ultrasonic Irradiation with Low Intensity on Protein Expressions of Caspase 12 and Apoptosis of HeLa Cells

秦娟;宋国林;王建三;林虹;刘卿;陈锦云;王嫣;
QIN Juan;SONG Guolin;WANG Jiansan;LIN Hong;LIU Qin;CHEN Jinyun;WANG Yan;Department of Gynaecology, Guiyang Maternal and Child Health Hospital;Department of ICU, 2nd Affiliated Hospital of Guizhou University of Traditional Chinese Medicine;State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing and the Ministry of Science and Technology, College of Biomedical Engineering, Chongqing Key Laboratory of Biomedical Engineering,Chongqing Collaborative Innovation Center for Mi

摘要(Abstract):

目的:探讨低强度超声辐照对人宫颈癌HeLa细胞凋亡及半胱氨酸天冬氨酸特异性蛋白酶-12(Casepase-12)蛋白表达的影响。方法:采用低强度超声辐照人子宫颈癌HeLa细胞株,于辐照后6及24 h时,Annexin V/PI双染法检测HeLa细胞的凋亡率,透射电子显微镜观察细胞的结构变化,Western blot法测定细胞匀浆中Caspase-12蛋白表达水平,免疫组织化学法检测Caspase-12蛋白在HeLa细胞中的阳性表达率。结果:低强度超声辐照后6 h时HeLa细胞的凋亡率、细胞Caspase-12蛋白表达水平及Caspase-12蛋白在HeLa细胞中的阳性表达率显著高于对照组,差异有统计学意义(P<0.05);辐照后24 h时变化更显著,差异有统计学意义(P<0.01);透射电子显微镜下可见,辐照后6 h时HeLa细胞内开始出现凋亡小体,24 h时胞浆浓缩、内质网变疏松并与胞膜融合、形成空泡;辐照后6 h及24 h时,Caspase-12蛋白在HeLa细胞胞浆中表达、呈棕黄色颗粒,但在对照组细胞中无表达。结论:低强度超声辐照可以诱导人宫颈癌HeLa细胞凋亡,并随着辐照后时间延长而增加,其机制可能与相关凋亡蛋白Caspase-12表达水平上调有关。
Objective: To study the effect of ultrasonic irradiation with low intensity on the protein expressions of Caspase 12 and apoptosis of HeLa cells. Methods: Human cervical cancer HeLa cells were cultured with ultrasonic irradiation of low intensity. Flow cytometry was used to detect the apoptosis rate in 6 and 24 hours after ultrasound irradiation. The structural changes of cells under ultrasound irradiation were observed by scanning electron microscope. The protein expression level of Caspase 12 in the cervical cancer cells was determined. Results: The apoptosis rate of HeLa cells 6 hours after ultrasound irradiation was significantly higher than that of the control group(P<0.05). The apoptosis rate increased in 24 hours after surgery, significantly higher than that of the control group(P<0.01). Apoptosis body in HeLa cells was induced in 6 hours after ultrasound irradiation. The expression of related apoptosis protein Caspase 12 was activated after ultrasound irradiation, compared with the control group(P<0.05). The expression of Caspase 12 in 24 hours after irradiation increased significantly compared with the control group(P<0.01). Conclusion: Low intensity ultrasound can induce apoptosis of HeLa cells and the apoptosis rate increases with the time of irradiation. Caspase 12 can be activated under ultrasound irradiation, and the expression of Caspase 12 increases with time of irradiation.

关键词(KeyWords): 超声多普勒,低强度;宫颈癌;HeLa细胞;细胞凋亡;凋亡蛋白;半胱氨酸天冬氨酸特异性蛋白酶-12;免疫组织化学法
ultrasound,low intensity;cervical cancer;HeLa cells;apoptosis;apoptosis protein;Caspase 12;immunohistochemistry

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81560287);; 贵州省科技厅基金[黔科合J字(2013)2020]

作者(Authors): 秦娟;宋国林;王建三;林虹;刘卿;陈锦云;王嫣;
QIN Juan;SONG Guolin;WANG Jiansan;LIN Hong;LIU Qin;CHEN Jinyun;WANG Yan;Department of Gynaecology, Guiyang Maternal and Child Health Hospital;Department of ICU, 2nd Affiliated Hospital of Guizhou University of Traditional Chinese Medicine;State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing and the Ministry of Science and Technology, College of Biomedical Engineering, Chongqing Key Laboratory of Biomedical Engineering,Chongqing Collaborative Innovation Center for Mi

DOI: 10.19367/j.cnki.1000-2707.2019.10.009

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