赵华,宗春燕,周振,曹毅
ZHAO Hua,ZONG Chunyan,ZHOU Zhen,CAO Yi

• 1:贵州医科大学公共卫生学院环境污染与疾病监控省部共建教育部重点实验室

摘要(Abstract)：

目的:观察不同时点900 MHz微波辐射暴露对雄性大鼠血浆褪黑素(Mel)和睾酮水平(T)昼夜节律的影响。方法:健康雄性SD大鼠110只,选取Mel水平昼夜节律差异无统计学意义的大鼠60只随机均分为对照组和照射组;两组大鼠再分别分为6个时点组(2∶00 AM、6∶00 AM、10∶00 AM、2∶00 PM、6∶00 PM、10∶00 PM)暴露,照射组大鼠给予2 000μW/cm~2 900 MHz的微波辐射、1 h/d、持续30 d,同时点对照组大鼠给与微波辐射假暴露;暴露结束后,各组大鼠每间隔4 h采血1次,测定血浆中的T和Mel水平,比较血浆T和Mel水平昼夜节律,分析微波辐射对血浆T和Mel水平影响的敏感时点。结果:照射组大鼠与对照组比较,2∶00 AM辐射暴露后Mel水平昼夜节律的峰值时点延迟937 h,与其它5个时点比较,其峰值时点变化最大;2∶00 PM辐射暴露后Mel水平昼夜节律的峰值时点延迟093 h,与其它5个时点相比,变化最小;6∶00 PM辐射暴露后T昼夜节律的峰值时点延迟1198 h,与其它5个时点比较,变化最大;10∶00 AM辐射暴露后T昼夜节律峰值时点延迟301 h,与其它5个暴露时点比较,变化最小。结论:不同时点2 000μW/cm~2 900 MHz微波辐射暴露对大鼠血浆T和Mel水平的敏感时点不同,以夜间影响较为明显。
Objective: To investigate the effects of 900 MHz microwave radiation at different time on circadian rhythm of melatonin, testosterone and estradiol levels in plasma of male rats.Methods: The circadian rhythm for locomotor activity and plasma melatonin levels of 110 SD adult male rats were measured, of which 60 rats were randomly divided into radiation group and control group with similar circadian rhythm of melatonin levels in plasma. Each large group was divided into 6 time points(2∶00 AM,6∶00 AM,10 ∶ 00 AM,2 ∶ 00 PM,6 ∶ 00 P M and 10 ∶ 00 PM). Rats in each radiation group were exposed to 2 000 μw/cm~2 900 MHz microwave radiation at different time, 1 h/d, 30 days at zeitgeber time(ZT). At the same time, control group rats were given false exposure to microwave radiation.After the end of exposure, blood samples were collected once every 4 h for each group and the plasma T and Mel levels were measured, the circadian rhythm of plasma T and Mel levels were compared, the sensitive time points of the effect of microwave radiation on plasma T and Mel levels were analyzed.Results: Compared with the control group, the peak time point of the circadian rhythm of Mel level after radiation exposure at 2∶00 AM was delayed 9.37 h, and compared with the other 5 time points,there was the greatest change at the peak time point. After radiation exposure at 2 ∶00 PM, the peak time point of circadian rhythm at Mel level was delayed 0.93 h with the smallest change compared to the other 5 time points. After radiation exposure at 6 ∶ 00 PM, the peak time point of T's circadian rhythm was delayed 11. 98 h with the largest change compared to the other 5 time points. After radiation exposure at 10∶00 AM, the peak time point of T circadian rhythm was delayed 3.01 h with the smallest change compared to the other 5 exposure time points.Conclusion: The effects of 2 000 μw/cm~2 900 MHz microwave radiation on plasma melatonin and testosterone levels are different when rats are exposed on different time points, and the effects are more obvious at night.

关键词(KeyWords)： 辐射损伤;微波;昼夜节律;时间因素;褪黑素;睾酮
radiation damage;microwave;circadian rhythm;time factor;melatonin;testosterone

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81373025)

作者(Author): 赵华,宗春燕,周振,曹毅
ZHAO Hua,ZONG Chunyan,ZHOU Zhen,CAO Yi

DOI: 10.19367/j.cnki.1000-2707.2020.02.006

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