杨志军,左丽,韦隆华,周永兵
YANG Zhi-jun~(1,2), ZUO Li~(1,2), WEI Long-hua~3, ZHOU Yong-bing~(1,2)(1.Department of Immunology, Guiyang Medical College, Guiyang 550004, China; The Center of Tissue Engineering and Stem Cell Research, Guiyang Medical College, Guiyang 550004, China; 2. Department of Microbiology, Guiyang Sanitation School, Guiyang 550020, China)

• 1:贵阳医学院免疫学教研室
• 2:贵阳医学院免疫学教研室
• 3:贵阳市卫校微生物学教研室
• 4:贵阳医学院免疫学教研室 贵州贵阳550004 贵阳医学院组织工程与干细胞实验中心

摘要(Abstract)：

目的:了解贵州省夏秋季不明原因发热病人中2型登革病毒的感染情况。方法:收集贵州省不同地区2002和2003年夏秋季不明原因发热病人血清364份,用间接酶联免疫吸附测定(ELISA)检测抗2型登革病毒特异性IgM,IgG类抗体。结果:病人血清抗2型登革病毒特异性IgG类抗体平均阳性率为12.6%,特异性IgM类抗体平均阳性率为18.4%,特异性IgG,IgM类抗体均阳性为4.7%。42份病人双份血清中,抗2型登革病毒特异性IgG类抗体效价增高4倍或以上为13份(31%);结论:贵州省夏秋季发热病人部分为2型登革病毒感染。
Objective: To investigate the status of DEN-2 infection in patients who got fever with uncertain reasons in summer and autumn in Guizhou. Methods: To analyze the special IgG and IgM kind antibodies against DEN-2 from 364 patients'serum from different areas of Guizhou province with indirect ELISA test. Results: The average positive rate of special IgG kind antibody against DEN-2 was 12.6%, special IgM kind antibody 18.4%, and the patients that have both antibodies together occupied 4.7%. Of the 42 positive specimens, 13(30.95%) had IgG antibody against DEN-2 with titre that increased at least 4 times. Conclusion: In Guizhou, some of the patients who got fever with uncertain reasons in summer and autumn might be infected by DEN type 2.

关键词(KeyWords)： 登革热病毒;登革出血热;登革热;酶联免疫吸附测定;贵州
dengue virus; dengue hemorrhagic fever; dengue; enzyme-linked immunosorbent assay;Guizhou

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(30360101);; 贵州省优秀科技教育人才省长资金资助项目(2003)

作者(Authors): 杨志军,左丽,韦隆华,周永兵
YANG Zhi-jun~(1,2), ZUO Li~(1,2), WEI Long-hua~3, ZHOU Yong-bing~(1,2)(1.Department of Immunology, Guiyang Medical College, Guiyang 550004, China; The Center of Tissue Engineering and Stem Cell Research, Guiyang Medical College, Guiyang 550004, China; 2. Department of Microbiology, Guiyang Sanitation School, Guiyang 550020, China)

DOI: 10.19367/j.cnki.1000-2707.2005.04.001

参考文献(References)：

• [1]Ddhain F.The situation of dengue in the world[J].Bu llSoc Pathol Exot,1996,89(2):87.
• [2]Q iu FX,Chen QQ,He QY,et al.The first ep idem ic of den-gue haemorrhagic fever in the Peop le’s Repub lic of Ch ina[J].Am J Trop M ed Hyg,1991,44(4):364.
• [3]Castleberry JS,M ahon CR.Dengue fever in the W esternHem isphere[J].C lin Lab Sc i,2003,16(1):34-38.
• [4]G ibbons,Robert V,Vaughn,et al.Dengue an escalatingprob lem[J].B ritish M edJ,2002,324(7353):1 563-1 566.
• [5]Hales S,De W etN,M aindonald J,et al.Potential effect ofpopu lation and c lim ate changes on global d istribution ofdengue fever:an emp irical model[J].Lancet,2003,361(9356):534.
• [6]B lack WC,BennettKE,Gorro EN,et al.F lavivirus suscep-tib ility in Aedes aegypti[J].Arch M ed Res,2002,33(4):379-388.
• [7]舒莉萍,左丽,郝牧,等.贵州自然界白纹伊蚊体内登革病毒带毒情况[J].中国公共卫生,2004,20(5):532-534.
• [8]舒莉萍,左丽,郝牧,等.从贵州白纹伊蚊体内分离到一株2型登革病毒[J].微生物学报,2004,44(2):153-156.

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