周诚,李竹,胡瑞洁,刘日光,叶川,贾湘谦,吴昌林,韩子翼
ZHOU Cheng,LI Zhu,HU Ruijie,LIU Riguang,YE Chuan,JIA Xiangqian,WU Changlin,HAN Ziyi

• 1:贵阳市第一人民医院
• 2:贵州医科大学
• 3:贵州医科大学附院

摘要(Abstract)：

目的:研究人骨髓间充质干细胞(BMSCs)与3-羟基丁酸酯-3-羟基己酸酯共聚物(PHBHHx)的生物相容性。方法:将PHBHHx(实验组)及明胶海绵(对照组)与第7代BMSCs复合培养7 d,通过光镜和扫描电镜分别观察BMSCs的生长及分布情况。结果:联合培养7 d,在显微镜下均可见两组材料周围有大量细胞生长,而材料内部及表面的细胞生长情况不能观察;电镜下见PHBHHx材料表面及孔隙内均有大量细胞附着,细胞相互连接融合成片,充满材料孔隙与表面,细胞表面可见大量的微绒毛,表示细胞生长状态良好,而明胶海绵上未见明显细胞生长。结论:PHBHHx具有良好的BMSCs相容性,可以作为一种新型的生物材料应用于组织工程。
Objective: To study the biocompatibility of bone marrow mesenchymal stem cells( BMSCs) and 3-hydroxybutyrate-co-3-hydroxyhexanoate copolyesters( PHBHHx). Methods: The PHBHHx( experimental group) and gelatin sponge( control group) were cultured with BMSCs of the seventh generation for 7 days,and the growth and distribution condition of BMSCs were observed by light microscopy and scanning electron microscopy. Results: After cultured for 7 days,under the microscope there were a large number of cells around the two materials,while inside and outside the material the cell growth cannot be observed; under electron microscope,attachment of a large number of cells were visible on the surface and in the pores of PHBHHx material,cells connected and were full of surface and pores,There are a lot of microvilli on cell surface,which showed cells in good condition,but no obvious cell growth were found in gelatin sponge. Conclusion: PHBHHx membrane has good BMSCs compatibility,and it can be used as a new biological materials in tissue engineering.

关键词(KeyWords)： 骨髓;间充质干细胞;组织工程;3-羟基丁酸酯-3-羟基己酸酯共聚物;明胶海绵;生物相容性
bone marrow;mesenchymal stem cells;tissue engineering;3-hydroxybutyrate-co-3-hydroxyhexanoate copolyesters;gelatin sponge;biocompatibility

Abstract:

Keywords:

基金项目(Foundation): 贵阳市科技局资助项目2014科技类计划[筑科合同(2014)100125号]

作者(Author): 周诚,李竹,胡瑞洁,刘日光,叶川,贾湘谦,吴昌林,韩子翼
ZHOU Cheng,LI Zhu,HU Ruijie,LIU Riguang,YE Chuan,JIA Xiangqian,WU Changlin,HAN Ziyi

DOI: 10.19367/j.cnki.1000-2707.2017.01.012

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