魏娟娟,史晓艳
WEI Juanjuan,SHI Xiaoyan

• 1:榆林市中医院检验科
• 2:榆林市星元医院检验科

摘要(Abstract)：

目的:探讨社区获得性肺炎(CAP)患者肺炎克雷伯菌耐药性及产β-内酰胺(AmpC)酶情况。方法:CAP患者72例,吸取痰液进行肺炎克雷伯菌培养筛选分离,采用K-B法检测肺炎克雷伯菌对8种抗菌药物的耐药性,聚合酶链式反应(PCR)检测肺炎克雷伯菌中AmpC酶基因的表达。结果:72例患者共检出100株细菌,肺炎克雷伯菌30株(30%);肺炎克雷伯菌对亚胺培南无耐药性,对头孢吡肟、阿米卡星的耐药率较低,而对青霉素类和第2、3代头孢类药物耐药率较高;在30株肺炎克雷伯菌中,检出AmpC阳性表达菌株11株(36.7%)。结论:肺炎克雷伯菌对青霉素类和第2、3代头孢类药物耐药,产AmpC酶基因检出率较高。
Objective: To investigate the drug resistance and producing AmpC enzyme gene conditions of Klebsiella pneumoniae of community-acquired pneumonia. Methods: Sputum from 72 CAP patients was collected for Klebsiella pneumoniae,and they were given eight different kinds of drug-resistant testing,while the AmpC enzyme gene expression were detected with PCR. Results: One hundred bacteria strains were detected from 72 patients,among them 30 strains( 30%) were Klebsiella pneumoniae. The Klebsiella pneumoniae strains showed no drug resistance to imipenem,and showed low resistance to cefepime and amikacin,and its resistance to penicillins,second and third generation cephalosporins were higher. In 30 Klebsiella pneumoniae strains,AmpC of 11( 36. 7%) strains were positive. Conclusion: Klebsiella pneumonia shows higher drug resistance to penicillins,second and third generation cephalosporins,producing AmpC enzyme gene rate is high.

关键词(KeyWords)： 社区获得性肺炎;克雷伯菌,肺炎;β-内酰胺酶类;耐药性
community-acquired pneumonia;Klebsiella pneumoniae;beta-lactamases;drug resistance

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 魏娟娟,史晓艳
WEI Juanjuan,SHI Xiaoyan

DOI: 10.19367/j.cnki.1000-2707.2015.09.019

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