周从容,李荣荣,赵淑云,罗锦
ZHOU Cong-rong; LI Rong-rong; ZHAO Shu-yun; LUO Jin (Department of Obstetrics and Gynecology;

• 1:贵阳医学院附院妇产科
• 2:贵阳医学院附院妇产科
• 3:贵阳医学院附院妇产科
• 4:贵阳医学院附院妇产科 贵州贵阳550004

摘要(Abstract)：

目的 :分析囊胚培养及移植技术在体外受精 -胚胎移植 (IVF ET)中的临床效果。方法 :将行IVF ET的 2 4例患者共 2 92个卵子采用Quin’s培养基 +10 %人血清白蛋白进行序贯培养及囊胚移植 ,与传统培养 D3(第3天 )移植进行比较 ,分析受精率、卵裂率、移植胚胎数及妊娠率 ,采用 χ2 检验进行统计学分析。结果 :囊胚培养组移植胚胎数明显低于传统培养组 (P <0 0 0 1) ,而妊娠率略有提高。并于 2 0 0 2年 2月 8日诞生了贵州省首例囊胚培养及移植的试管婴儿龙凤胎。结论 :采用适合的培养液进行囊胚培养 ,可减少胚胎移植数目 ,提高IVF ET的妊娠成功率 ,降低多胎率。
Objective: To study the clinical effects of blastocyst culture technique on the outcome of in vitro fertilization-embryo transfer(IVF-ET). Methods: 292 ova from 24 patients were fertilized and sequentially cultured in Quin’s media plus 10% human serum albumin(HSA)in vitro. In 24 IVF-ET cycles, transplantation was performed at the blastocyst stage. Conventional culture that the transplantation was done on the third day after fertilization was carried out in 28 IVF-ET cycles. The fertilization rate, cleavage rate, the numbers of embryo transfered (NET) and the pregnancy rate were compared between these two groups. Results: The NET were significantly smaller in blastocyst culture group than those in conventional culture group(P<0.001). Conclusion: Blastocyst culture is more suitable for selecting good embryos to transfer, which can increase the pregnancy rate and decrease the multiple pregnancy rate.

关键词(KeyWords)： 胚泡;受精,体外;胚胎移植
blastocyst; fertilization in vitro; embryo transfer

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 周从容,李荣荣,赵淑云,罗锦
ZHOU Cong-rong; LI Rong-rong; ZHAO Shu-yun; LUO Jin (Department of Obstetrics and Gynecology;

DOI: 10.19367/j.cnki.1000-2707.2004.02.009

参考文献(References)：

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• [2]ScholtesMC ,ZeilmakerGH .Aprospective,randomizedstudyofembryotransferresultsafter3or5daysofembryocultureinvitrofertilization[J].FertilSteril,1996,65:1245.
• [3]LangleyMT ,MarekDM ,GardnerDK ,etal.Extendedembryocultureinhumanassistedreproductiontreatments[J].HumReprod,2001,(16):902.
• [4]ShapiroBS ,HarrisDC ,RichterKS .Predictivevalueof72 hourblastomerecellnumberonblastocystdevelopmentandsuccessofsubsequenttransferbasedonthedegreeofblastocystdevel opment[J].FertilSteril,2000,73:582.

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