杨健,李靖,彭潇,董莉,沈艳珍,李勇军,席晓岚,刘亭
YANG Jian,LI Jing,PENG Xiao,DONG Li,SHEN Yanzhen,LI Yongjun,XI Xiaolan,LIU Ting

• 1:贵州医科大学贵州省药物制剂重点实验室/省部共建药用植物功效与利用国家重点实验室
• 2:贵州医科大学药学院
• 3:贵州医科大学民族药与中药开发应用教育部工程研究中心
• 4:贵州维康子帆药业股份有限公司

摘要(Abstract)：

目的:研究骨康胶囊组方药物续断、三七、芭蕉根对体外培养人成骨细胞Sa OS-2增殖、分化和矿化的影响。方法:以不含药培养基培养Sa OS-2作为对照组,给药组Sa OS-2分别用1、10、100、200、400、800 mg/L续断、芭蕉根及三七水提物作用,MTT法测定Sa OS-2细胞增殖情况,磷酸苯二钠比色法检测碱性磷酸酶(ALP)活性,ELISA法检测钙离子和骨钙素(OTC)的分泌以及用茜素红染色法测定成骨细胞矿化结节数。结果:与对照组比较,续断各浓度水提物可显著促进Sa OS-2细胞的增殖、增强ALP活性且对细胞Ca2+的分泌有促进作用(P<0. 05或P <0. 01),浓度为100、200 mg/L的续断水提物对Sa OS-2细胞分泌OTC能力及对细胞的矿化有促进作用(P <0. 05或P <0. 01);三七水提物除了浓度为200 mg/L时有明显促进OTC分泌的作用(P <0. 05)外,浓度为10、100 mg/L的三七水提物及各浓度的芭蕉根水提物对成骨细胞增殖、分化和矿化作用均不明显(P>0. 05)。结论:骨康胶囊治疗骨质疏松作用可能与其组方续断的促进成骨细胞的增殖、分化及矿化的作用有关。
Objective: To investigate in vitro effects of components( including Dipsacus asper,Panax notoginseng,Musa basjoo Sieb) of Gu Kang capsule on cell proliferation,differentiation and mineralization of human osteoblast SaOS-2 cells. Methods: SaOS-2 cells were cultured and treated with different concentrations of water extracts of Dipsacus asper,Panax notoginseng,Musa basjoo Sieb. Cell proliferation was determined using MTT assay. Alkaline phosphatase( ALP) activity was measured by benzene disodium phosphate colorimetriy,and secretions of Ca2 +,osteocalcin( OTC) were detected by ELISA assay. Osteoblast mineralization nodules were measured by alizarin red staining. Results: Compared with untreated group,water extract of Dipsacus asper at 10,100 and 200 mg/L concerntrations significantly promoted the proliferation of osteoblasts,enhanced ALP activity and Ca2 +secretion in vitro( P< 0. 05,P < 0. 01). Water extract of Dipsacus asper at 100 and 200 mg/L concerntrations could promote the secretion of OTC and osteoblasts mineralization( P < 0. 05,P < 0. 01),while its effects were not obvious at 10 mg/L concentration( P > 0. 05). Water extract of Panax notoginseng at 200 mg/L concerntration could significantly promote OTC secretion( P < 0. 05). However,water extract of Panax notoginseng at 10 and 100 mg/L showed no significant effect on the proliferation,differentiation and mineralization of SaOS-2 cells( P > 0. 05). Furthermore,water extract of Musa basjoo Sieb had no significant effect on proliferation,differentiation and mineralization of SaOS-2 cells. Conclusions: The anti-osteoporosis effect of Gukang Capsule may be associated with its roles of Dipsacus asper in promoting the proliferation,differentiation and mineralization of osteoblasts SaOS-2.

关键词(KeyWords)： 骨康胶囊;续断;三七;芭蕉根;成骨细胞;增殖;分化;矿化;碱性磷酸酶;骨钙素
Gukang capsule;Dipsacus asper;Panax notoginseng;Musa basjoo Sieb;osteoblast;proliferation;differentiation;mineralization;alkaline phosphatase;osteocalcin

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技计划项目[黔科合J重大字(2015)2002、黔科合LH字(2016)7373];; 贵州省教育厅项目[黔教合重大专项字(2015)040];; 贵州省创新人才团队项目[黔科合平台人才(2016)5613、黔科合平台人才(2016)5677]

作者(Author): 杨健,李靖,彭潇,董莉,沈艳珍,李勇军,席晓岚,刘亭
YANG Jian,LI Jing,PENG Xiao,DONG Li,SHEN Yanzhen,LI Yongjun,XI Xiaolan,LIU Ting

DOI: 10.19367/j.cnki.1000-2707.2019.02.007

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