许键炜,何志旭,舒莉萍,王凤昌
XU Jianwei,HE Zhixu,SHU Liping,WANG Fengchang

• 1:贵阳医学院组织工程与干细胞实验中心
• 2:贵阳医学院药理学教研室
• 3:贵阳医学院附院儿科
• 4:贵阳医学院免疫学教研室

摘要(Abstract)：

目的:观察人脐带间充质干细胞对裸鼠的致瘤性。方法:培养扩增人脐带来源的间充质干细胞,制备成细胞生理盐水混悬液注射裸鼠。观察8周裸鼠体内有无肿瘤生长。结果:裸鼠注射细胞后无明显不适,8周观察期内裸鼠体内未见肿瘤生长,病理组织学检测未见异常。结论:人脐带间充质干细胞导致肿瘤发生的可能性低。
Objective: To investigate nude mice's tumorigenicity caused by human umbilical cord mesenchymal stem cells( HUCMSCs). Methods: Cultured and amplified HUCMSCs,and prepared into cell normal saline hypodermic injection used for injecting nude mice. Tumor growth condition was observed for eight weeks. Results: Mice had no obvious adverse reaction,8 weeks of tumorigenicity study showed that no tumor developed. Histopathologic examinations also showed no abnormality. Conclusions: The possibility of HUCMSCs lead to tumor growth is very low.

关键词(KeyWords)： 脐带;间充质干细胞;细胞,培养的;小鼠,裸;免疫组织化学;致癌性试验
umbilical cord;mesenchymal stem cells;cell,cultivated;mice,nude;immunohisto-chemistry;tumorigenicity test

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(No.30560159);; 贵州省“125计划”重大科技专项[黔教合重大专项字(2013020)];; 贵州省教育厅自然科学研究项目[黔教合KY字(2012036)]

作者(Author): 许键炜,何志旭,舒莉萍,王凤昌
XU Jianwei,HE Zhixu,SHU Liping,WANG Fengchang

DOI: 10.19367/j.cnki.1000-2707.2015.01.005

参考文献(References)：

• [1]李忠俊,相丽欣,叶兴德.间充质干细胞研究进展[J].中国输血杂志,2013(4):307-309.
• [2]王艳,张晋林,黄晓兵,等.脐带间充质干细胞研究现状与临床治疗[J].重庆医学,2013(18):2161-2163.
• [3]Kalwitz G,Endres M,Neumann K,et al.Gene expression profile of adult human bone marrow-derived mesenchymal stem cells stimulated by the chemokine CXCL7[J].Int J Biochem Cell Biol,2009(3):649-658.
• [4]李六金,李秦,施新猷,等.五种动物传代细胞系对裸鼠的致瘤性[J].第四军医大学学报,2000(6):738-740.
• [5]Sell S.Stem cell origin of cancer and differentiation therapy[J].Crit Revoncol Hematol,2004(1):1-28.
• [6]国家药典委员会编写组.中华人民共和国药典(三部)[S].北京:中国医药科技出版社,2010:14-17.
• [7]Miyahara Y,Nagaya N,Kataoka M,et al.Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction[J].Nat Med,2006(4):459-465.
• [8]许键炜,申长清,赵芳芳,等.同源异体间充质干细胞上清液干预大鼠佐剂性关节炎研究[J].贵阳医学院学报,2013(4):339-343.
• [9]Zhou CH,Yang B,Tian Y,et al.Immunomodulatory effect of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells on Iymphocytes[J].Cell Immunol,2011(1):33-38.
• [10]崔冬冰,何志旭,李永念,等.人羊膜间充质干细胞的分离与鉴定[J].贵阳医学院学报,2014(1):8-11.
• [11]Hua JL,Qi u BP,Zhu HJ,et al.Multipotent mesenchymal stem cells(MSCs)from humanumbilical cord:Potential differentiation of germ cells[J].A Fric J of Biochem Res,2011(4):113-123.
• [12]Zhang J,Chen GH,Wang YW,et al.Hydrogen peroxide preconditioning enhances the therapeutic efficacy of Wharton’s Jelly mesenchymal stem cells after myocardial infarction[J].Chin Med J,2012(19):3472-3478.
• [13]Houghton JM,Stoicov C,Nomura S,et al.Gastric cancer originating from bone marrow-derived cells[J].Science,2004(5701):1568-1571.
• [14]Matsubara H.Risk to the coronary arteries of in tracoronary stem cell infus ion and GC SF cytokine therapy[J].Lancet,2004(9411):746-747.
• [15]Kuroda Yasuda S,Sato Y.Tumorigenicity studies for human pluripotent stem cell-derived product[J].Biol Pharm Bull,2013(2):189-192.
• [16]聂德志,宛莹,贲亮,等.Balb/c裸鼠体内的干细胞致瘤实验[J].中国组织工程研究,2014(6):888-893.

文章评论(Comment)：