骆姝琳;袁军;安宇;陈琨;任淩雁;令狐克燕;李伟;张春林;
LUO Shulin;YUAN Jun;AN Yu;CHEN Kun;REN Lingyan;LINGHU Keyan;LI Wei;ZHANG Chunlin;Central Laboratory,Guizhou Province People's Hospital;Department of Biology,Guizhou Medical University;

• 1:贵州省人民医院中心实验室
• 2:贵州医科大学生物学教研室

摘要(Abstract)：

目的:探讨染色体异常与贵州地区习惯性流产的关系。方法:采集就诊于贵州省某3级甲等医院的237对习惯性流产夫妇外周血,进行淋巴细胞培养,G显带,染色体核型分析。结果:273对(546例)习惯性流产的夫妇中异常染色体的检出率为8.60%(47/546),其中染色体平衡易位7例、罗伯逊易位3例及多态性变异37例,总异常率分别为14.89%(7/47)、6.38%(3/47)及78.73%(37/47);多态性变异中包括16例Y染色体异染色质增长;6例1号、9号、16号染色体次缢痕增长,15例D组和G组短臂随体变异。结论:染色体异常是引起贵州地区习惯性流产的重要病因之一,有必要对有习惯性流产史的夫妇双方进行异常染色体分析。
Objective: To investigate the correlation between chromosomal abnormality and recurrent spontaneous abortions( RSA) couples in Guizhou province. Methods: Chromosomal karyotypes were analyzed in 273 couples with RSA by G- banding after periphery blood lymphocyte culture. Results:Totally 47 abnormal chromosome karyotypes were found in 273 couples( 546 cases),7 cases of chromosome balance trans location,3 cases of Robertson translocation and 37 cases of abnormal polymorphism,and abnormal detection rate was 14. 89%( 7 /47),6. 38%( 3 /47) and 78. 73%( 37 /47). Of all the polymorphism variation cases,16 cases had heterochromatin growth of Y chromosome,6 cases of secondary constriction increase occurred on 1,9,16 chromosome,15 cases had D and G group chromosome with short arm body variation. Conclusions: The chromosome abnormality may be an important cause of RSA. Therefore,it is essential to analyze chromosomal karyotypes for RSA couples.

关键词(KeyWords)： 流产,习惯性;细胞遗传学分析;染色体核型;易位(遗传学)
abortion,recurrent;cytogenetic analysis;chromosome karyotype;translocation(genetics)

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅科技联合基金[黔科合SY(2010)3132号];; 贵州省卫生厅科学技术基金(gzwkj2012-1-006);; 贵阳市科技局社会发展与民生科技计划[筑科合同(2011103)号]

作者(Authors): 骆姝琳;袁军;安宇;陈琨;任淩雁;令狐克燕;李伟;张春林;
LUO Shulin;YUAN Jun;AN Yu;CHEN Kun;REN Lingyan;LINGHU Keyan;LI Wei;ZHANG Chunlin;Central Laboratory,Guizhou Province People's Hospital;Department of Biology,Guizhou Medical University;

DOI: 10.19367/j.cnki.1000-2707.2015.11.013

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