贵州医科大学学报

2015, v.40;No.172(01) 68-71+75

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强直性脊柱炎患者不同活动期Treg、Th17细胞的临床意义及其相关性
A Study on the Clinical Value and Correlation of Treg and Th17 Cells among Different Active Stages of Ankylosing Spondylitis

王作龙;钟乃风;马莉;
WANG Zuolong;ZHONG Naifeng;MA Li;Central Laboratory,Affiliated Hospital of Guiyang Medical College;Department of Biotechnology,School of Medical Bioengineering of Guiyang Medical College;

摘要(Abstract):

目的:探讨强直性脊柱炎(AS)不同活动期调节性T细胞(Treg)和辅助性T细胞17(Th17)的临床意义,分析两者之间的相关性。方法:78例AS患者(AS组)分为低活动性AS组和高活动性AS组,30例健康体检者作为对照组,抽取两组受检者外周血,应用细胞内因子染色技术及流式细胞术检测外周血Treg和Th17细胞数量,分析Treg和Th17与AS活动性的相关性。结果:与对照组相比,AS组和低活动性AS组外周血Treg细胞百分率的差异无统计学意义(P>0.05),高活动性AS组外周血Treg细胞百分率明显降低(P<0.01),AS组外周血Th17细胞百分率增高(P<0.01),且低活动性AS组和高活动性AS组外周血Th17细胞百分率均增高(P<0.05),AS组、低活动性AS组以及高活动性AS组外周血Th17/Treg的细胞比值均有增高(P<0.01);与低活动性AS组相比,高活动性AS组外周血Treg细胞明显减少(P<0.01),高活动性AS组外周血Th17细胞明显增高(P<0.01),高活动性AS组外周血Th17/Treg的细胞比率明显增高(P<0.01);相关性分析显示,AS患者外周血Th17细胞比例与Bath强直性脊柱炎病情活动指数(BASDI)呈正相关,(r=0.409)Treg细胞比例与BASDI呈负相关(r=-0.265),Th17/Treg比率与BASDI相关性分析呈显著正相关(r=0.459,P<0.01)。结论:AS患者外周血Treg、Th17细胞数量的改变,与AS的发生、发展有明显相关性,检测AS患者外周血Treg、Th17细胞的数量以及Thl7/Treg的比率有助于AS病情的判断。
Objective: To investigate the clinical significance and correlation of T helper cells 17( Th17) and regulatory T cells( Treg) in different stages of patients with ankylosing spondylitis( AS).Methods: A total of 78 peripheral blood samples were taken from AS patients,the AS group was divided into low-activity AS group and high-activity AS group,30 healthy peripheral blood samples serves as control group. Th17 and Treg numbers were detected by flow cytometry( FCM) and intracellular cytokine staining technique( ICS) to study the relationship between Treg,Th17 cells and AS activity. Results: Compared with the control group,the percentage of Treg cells in AS group and low-activity AS group had no significant difference( P > 0. 05),high-activity AS group peripheral blood Treg cells were significantly reduced( P < 0. 01). The percentage of Th17 cells in AS group increased( P <0. 01),peripheral blood Th17 percentage of low-activity AS group and high-activity AS group were increased( P < 0. 05),and the ratios of Th17 / Treg in AS group,low-activity AS group and high-activity AS group were increased( P < 0. 01). The ratio of Treg cells of high-activity AS group was significantly lower than that of low-activity AS group( P < 0. 01); the ratio of Th17 cell of high-activity AS group was significantly higher than that of low-activity AS group( P < 0. 01); the ratio of peripheral blood Th17 / Treg cell of high-activity AS group was significantly higher than that of low-activity AS group( P< 0. 01). There were positive correlation of Bath ankylosing spondylitis disease activity index( BASDI) with peripheral blood Th17 cell ratio and Th17 / Treg ratio of AS patients,negative correlation between BASDI and Treg cells ratio of AS patients( P < 0. 05). Conclusions: Changes of the amount of Treg and Th17 cells is related to the occurrence and development of AS. The amount of Treg and Th17 and the ratio of Th17 / Treg can contribute to diagnose AS patient condition and serve as the treatment target of AS.

关键词(KeyWords): 辅助性T细胞17;调节性T细胞;脊柱炎,强直性;免疫,细胞;自身免疫疾病
T helper cells 17;regulatory T cells;spondylitis,ankylosing;immunity,cellular;auto-immune diseases

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅社会发展攻关项目[黔科合LG字(2011)034号]

作者(Author): 王作龙;钟乃风;马莉;
WANG Zuolong;ZHONG Naifeng;MA Li;Central Laboratory,Affiliated Hospital of Guiyang Medical College;Department of Biotechnology,School of Medical Bioengineering of Guiyang Medical College;

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DOI: 10.19367/j.cnki.1000-2707.2015.01.018

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