陈美源,肖杰,江建新,喻超,张宏,陈玲,孙诚谊
CHEN Meiyuan,XIAO Jie,JIANG Jianxin,YU Chao,ZHANG Hong,CHEN Ling,SUN Chengyi

• 1:贵阳医学院附院肝胆外科

摘要(Abstract)：

目的:探讨microRNA-137(miR-137)对胰腺癌细胞侵袭和迁移能力的影响。方法:构建miR-137慢病毒表达载体(LV-miR-137)及空载体,将细胞分为3组:LV-miR-137感染组(实验组)、空载体组(阴性对照组)及空白组;LV-miR-137感染胰腺癌PANC-1和MIAPaCa2细胞后72h,实时荧光定量PCR(qRT-PCR)检测感染效率,Transwell小室侵袭实验及细胞划痕实验检测miR-137对PANC-1和MIAPaCa2细胞株侵袭和迁移能力的影响。结果:LV-miR-137感染胰腺癌细胞株PANC-1和MIAPaCa2后miR-137表达水平量明显升高,Transwell小室细胞侵袭实验发现:实验组PANC-1和MIAPaCa2细胞迁移能力明显受到抑制,细胞迁移数目(58.2±1.1,37.5±1.8)较空白对照组(141.7±7.9,74.2±2.4)和阴性对照组(151.7±5.8,72.2±2.9)明显减少,P<0.05,差异有统计学意义;PANC-1细胞的侵袭能力也明显受到抑制,细胞侵袭数目(44.0±1.5)较空白对照组(110.9±6.4)和阴性对照组(117.2±1.9)明显减少,P<0.05,差异有统计学意义;细胞划痕实验检测发现,24h后实验组胰腺癌MIAPaCa-2、PANC-1细胞的迁移距离明显比阴性对照组及空白组短,P<0.05,差异有统计学意义。结论:MiR-137能抑制胰腺癌细胞的侵袭和迁移能力,有望成为胰腺癌基因治疗的新靶点。
Objective: To investigate the effect of miR-137 on invasion and metastasis of human pancreatic cancer cells. Methods: miR-137 lentiviral vector( LV-miR-137) and the empty vector were constructed,the cells were divided into three groups: LV-miR-137 infected group( experimental group),empty vector( negative control group) and control group. After PANC-1 and MIA PaCa2 cells were infected with LV-mi R-137 for 72 h,real time quantitative PCR( q RT-PCR) was applied to detect the infection efficiency. The Transwell chamber assay and cell wound healing were employed to observe the effects of LV-miR-137 on invasion and metastasis of MIA PaCa2 and PANC-1 cell lines.Results: After infected with LV-miR-137,the mi R-137 expression levels in MIA PaCa2 and PANC-1cells increased. The Transwell chamber assay found that the migration ability of MIA Pa Ca2 and PANC-1 cells in experimental group was significantly inhibited,the cell migration number( 37. 5 ± 1. 8,58. 2± 1. 1) was obviously lower than that of control group( 74. 2 ± 2. 41,141. 7 ± 7. 9) and negative control group( 72. 2 ± 2. 91,151. 7 ± 5. 8),P < 0. 05. PANC-1 cell invasion ability was also significantly inhibited,the number of cell invasion( 44. 0 ± 1. 5) was lower than that of control group( 110. 9 ±6. 4) and negative control group( 117. 2 ± 1. 9),P < 0. 05. Wound-healing assay showed that MIA PaCa-2,PANC-1 cell migration distance was shorter than that of negative control group and control group after 24 h experiment,P < 0. 05. Conclusion: Mi R-137 can inhibit pancreatic cancer cell migration and invasion,which may be a new target for gene therapy of pancreatic cancer.

关键词(KeyWords)： 胰腺肿瘤;侵袭;迁移;微小RNA
pancreatic neoplasms;metastasis;invasion;microRNA

Abstract:

Keywords:

基金项目(Foundation): 国家国际科技合作专项资助(2014DFA31420);; 国家自然科学基金资助项目(81160311);; 贵州省科技厅项目[黔科合(2010)3149];; 贵州省科技厅联合基金计划项目[黔科合LH字(2014)7129]

作者(Author): 陈美源,肖杰,江建新,喻超,张宏,陈玲,孙诚谊
CHEN Meiyuan,XIAO Jie,JIANG Jianxin,YU Chao,ZHANG Hong,CHEN Ling,SUN Chengyi

DOI: 10.19367/j.cnki.1000-2707.2015.02.003

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