高楠楠;侯胜开;屈璐;潘艳伶;
GAO Nannan;HOU Shengkai;QU Lu;PAN Yanling;Department of Traditional Chinese Medicine, School of Clinical Medicine, Guizhou Medical University;Guizhou Provincial Key Laboratory of Pathogenesis & Grug Research on Common Chronic Diseases, Guizhou Medical University;

• 1:贵州医科大学临床医学院中医学教研室
• 2:贵州医科大学贵州省常见慢性疾病发病机制及药物研究重点实验室

摘要(Abstract)：

目的:探讨糖通饮对早期糖尿病肾病大鼠肾脏组织的保护作用。方法:雄性SD大鼠55只,随机分为正常组(A组)10只,剩余的45只造模,将造模成功的40只SD大鼠分为模型组(B组)、糖通饮组(C组)、厄贝沙坦组(D组)及糖通饮+厄贝沙坦组(E组),每组10只;C、D、E组给予对应的药物灌胃治疗,A组及B组给予等量生理盐水灌胃,8周末检测大鼠的空腹血糖(FBG)、血清肌酐(SCr)及24 h尿蛋白定量,取右侧肾脏苏木精-伊红(HE)染色光镜下观察组织学改变。结果:与A组比较,B组大鼠肾脏病理形态变化明显,FBG、SCr及24 h尿蛋白明显升高,差异有统计学意义(P<0.05);与B组比较,C、E组的FBG、SCr及24 h尿蛋白明显降低,D组的SCr及24 h尿蛋白明显降低,差异有统计学意义(P<0.05);与B组比较,C、D、E组治疗后病理改变均明显减轻。结论:糖通饮能够有效地减少早期DN大鼠的24 h尿蛋白,降低FBG和SCr水平,对大鼠肾脏组织具有保护作用。
Objective: To investigate the protective effects of Tangtong Drink on kidney tissue of rats with early diabetic nephropathy. Methods: A total of 55 male Sprague-Dawley rats were randomly divided into normal(A) group(n=10), and the remaining 45 rats were modeled. 40 SD rats with successful modeling were divided evenly into model(B) group, Tangtong Drink(C) Group, Irbesartan(D) group, and Tangtong Drink+Irbesartan(E) group. Group C, D, and E were given the corresponding drugs for intragastric administration, and group A and group B were given the same amount of normal saline. After 8 weeks, the fasting blood glucose(FBG), serum creatinine(SCr) and 24 h urine protein were measured. The pathological changes of rat kidney were observed under hematoxylin-eosin(HE) staining. Results: Compared with group A, the pathological changes of kidney in group B were significantly changed. FBG and SCr and 24 h urine protein significantly increased, and the differences were statistically significant(P<0.05). Compared with group B, FBG, SCr and 24 h urinary protein in group C and E significantly decreased, and SCr and 24 h urine protein significantly lower in group D. The differences were statistically significant(P<0.05). Compared with group B, the pathological changes were all significantly reduced in group C, D, and E. Conclusion: Tangtong Drink could effectively reduce the levels of FBG and SCr in rats with diabetic nephropathy and reduce 24 h urine protein, which showed protective effects of kidney tissues in rats.

关键词(KeyWords)： 糖尿病肾病;糖通饮;厄贝沙坦;尿蛋白;血清肌酐;病理学
diabetic nephropathy;Tangtong drink;irbesartan;urine protein;serum creatinine;pathology

Abstract:

Keywords:

基金项目(Foundation): 贵阳市人民政府-贵州医科大学科学技术联合基金(GY2015-21)

作者(Authors): 高楠楠;侯胜开;屈璐;潘艳伶;
GAO Nannan;HOU Shengkai;QU Lu;PAN Yanling;Department of Traditional Chinese Medicine, School of Clinical Medicine, Guizhou Medical University;Guizhou Provincial Key Laboratory of Pathogenesis & Grug Research on Common Chronic Diseases, Guizhou Medical University;

DOI: 10.19367/j.cnki.1000-2707.2019.06.011

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