贵州医科大学学报

2006, (03) 232-234

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整合素β1、α2亚基在培养大鼠椎间盘纤维环细胞中的表达
Expression of Integrin β1 and α2 Subunits in Cultured Annulus Fibrosus Cells of Rat Intervertebral Disc

郭志良;周跃;成敏;李华壮;曹国永;
GUO Zhi-liang,ZHOU Yue,CHENG Min,LI Hua-zhuang,CAO Guo-yong(Department of Orthopedics,Xinqiao Hospital,The Third Military Medical University,Chongqing 400037,China)

摘要(Abstract):

目的:检测培养大鼠椎间盘纤维环细胞整合素β1、α2亚基的表达。方法:用酶消化法分离、培养大鼠椎间盘纤维环细胞,采用免疫荧光细胞化学染色和流式细胞术检测培养细胞整合素β1、α2亚基的表达,并对其进行定量分析。结果:培养大鼠椎间盘纤维环细胞表达整合素β1、α2亚基,整合素β1表达量较高,α2亚基表达较低。结论:培养大鼠椎间盘纤维环细胞表达整合素β1、α2亚基。
Objective: To investigate whether the integrin β1 and α2 subunits are expressed in rat annulus fibrosus cells.Methods: The rat annulus fibrosus cells were isolated and cultured in vitro.Immunocytochemistry and flow cytometry were used to detect the integrin β1 and α2 subunits in cultured cells.Quantitative analysis of the integrin subunits were carried out by flow cytometry.Results: The results showed that integrin β1 and α2 subunits expressed in cultured annulus fibrosus cells.The β1 subunit was more abundant than α2 subunit.Conclusions: The results provide evidence that the integrin β1 and α2 subunits are expressed in the rat annulus fibrosus cells cultured in vitro.

关键词(KeyWords): 整合素;椎间盘;细胞,培养的;大鼠
intervertedrol disk;cells,cultured;rats

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作者(Author): 郭志良;周跃;成敏;李华壮;曹国永;
GUO Zhi-liang,ZHOU Yue,CHENG Min,LI Hua-zhuang,CAO Guo-yong(Department of Orthopedics,Xinqiao Hospital,The Third Military Medical University,Chongqing 400037,China)

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DOI: 10.19367/j.cnki.1000-2707.2006.03.011

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