游赣花,罗俊,张学愈,潘年松,张珏,张卫霞,涂铭旌,盛勇
YOU Ganhua,LUO Jun,ZHANG Xueyu,PAN Niansong,ZHANG Jue,ZHANG Weixia,TU Mingjing,SHENG Yong

• 1:贵州省食品药品检验所药理毒理室
• 2:贵州医科大学药理教研室
• 3:遵义医药高等专科学校天然药物教研室
• 4:四川大学材料学院

摘要(Abstract)：

目的:探讨姜黄素对宫颈癌Hela细胞荷瘤裸鼠的抑瘤作用。方法:40只雌性裸鼠腋下接种Hela细胞构建荷瘤模型,随机分为姜黄素低、中、高剂量组、5-氟尿嘧啶(5-Fu)组及模型组,分别腹腔注射压榨法提取姜黄素60、120、240 mg/kg,5-Fu 20 mg/kg及等量生理盐水,0.2 m L/只,隔天1次,连续给药10次;观察肿瘤生长情况、抑瘤率、病理组织学改变,检测细胞周期及凋亡率。结果:姜黄素低、中、高剂量组的抑瘤率分别为20.14%,32.43%,59.56%,呈剂量依赖性升高;病理切片可见姜黄素给药组细胞核固缩、裂解,肿瘤组织内有炎性细胞浸润,纤维结缔组织增生明显,血管数量减少;姜黄素各给药组的肿瘤细胞凋亡率为12.79%,16.23%,21.90%,较模型组明显增加(P<0.05),且肿瘤细胞主要被阻滞于G2/M期。结论:压榨法提取姜黄素可显著抑制宫颈癌裸鼠移植瘤的增殖,其机制可能与姜黄素诱导癌细胞凋亡有关。
Objective: To investigate the anti-tumor effects of curcumin on nude mice with cervical cancer. Method: Forty nude female mice were inoculated Hela cells in axillary skin,and dividing into five groups randomly: the low,medium,high dose group of curcumin( 60 mg / kg,120 mg / kg,240 mg / kg),the 5-Fu group and the model group. Then,injecting the curcumin,which from pressing60,120,240 mg / kg,5-Fu 20 mg / kg and equal amount of normal saline into mice with intraperitoneal injection way,0. 2 m L for each at the frequency of one time every other day for 10 times. The tumor growth,anti-tumor rate and histopathology were observed. Meanwhile,the cell cycle and apoptosis rate were also detected. Results: The anti-tumor rates of the low,medium,high dose groups of curcumin were 20. 14%,32. 43%,59. 56% respectively,which indicated dose-dependent; according to the results of pathological section,these groups curcumin cell nucleus pyknosis,lysis,meanwhile the inflammatory cell infiltration in tumor tissue,and the proliferation of fibrous connective tissue was significantly,however the number of blood vessels decreased. The flow cytometry results showed that the tumor cell apoptosis rates of the low,medium,high dose groups of curcumin were 12. 79%,16. 23%,21. 90%. Comparing with the model group,they were increased significantly( P < 0. 05),and most of tumor cell was blocked in the G2 / M phase. Conclusion: Curcumin from pressing can inhibit the cervical cancer proliferation in nude mice significantly,the mechanism may be rlated to the apoptosis of cancer cells induced by curcumin.

关键词(KeyWords)： 姜黄素;宫颈肿瘤;癌;裸鼠;凋亡
curcumin;cervix neoplasms;carcinoma;nude mice;apoptosis

Abstract:

Keywords:

基金项目(Foundation): 贵州省教育厅自然科学研究项目[黔教科(2009)0101号];; 贵州省科技计划项目[黔科合SY(2008)3027]

作者(Author): 游赣花,罗俊,张学愈,潘年松,张珏,张卫霞,涂铭旌,盛勇
YOU Ganhua,LUO Jun,ZHANG Xueyu,PAN Niansong,ZHANG Jue,ZHANG Weixia,TU Mingjing,SHENG Yong

DOI: 10.19367/j.cnki.1000-2707.2015.09.006

参考文献(References)：

• [1]Jemal A,Bray F,Center MM,et al.Global cancer statistics[J].CA Cancer Clin,2011(2):869-901.
• [2]Wei X,Zhou D,Wang H,et al.Effects of pyridine analogs of curcumin on growth,apoptosis and NF-κB activity in prostate cancer PC-3 cells[J].Anticancer Res,2013(4):1341-1350.
• [3]Xi Y,Gao H,Callaghan MU,et al.Induction of BCL2-Interacting Killer,BIK,is mediated for anti-cancer activity of curcumin in human head and neck squamous cell carcinoma cells[J].J Cancer,2015(4):327-332.
• [4]Li Q,Chen J,Luo S,et al.Synthesis and assessment of the antioxidant and antitumor properties of asymmetric curcumin analogues[J].Eur J Med Chem,2015(3):461-469.
• [5]Kerr JF,Wyllie AH,Currie AR.Apoptosis:a basic biological phenomenon with wide ranging implication in tissue kinetics[J].Br J Cancer,1972(4):239-257.
• [6]Kamesaki H.Mechanisms involved in chemotherapy-induced apoptosis and their implications in cancer chemotherapy[J].Int J Hematol,1998(1):29-43.
• [7]Yang L,Cui Y,Shen J,et al.Antitumor activity of SA12,a novel peptide,on SKBr-3 breast cancer cells via the mitochondrial apoptosis pathway[J].Drug Des Devel Ther,2015(9):1319-1330.
• [8]Choi DW,Lim MS,Lee JW,et al.The cytotoxicity of Kahweol in HT-29 human colorectal cancer cells is mediated by apoptosis and suppression of heat shock protein 70 expression[J].Biomol Ther(Seoul),2015(2):128-133.

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