王作龙,钟乃风,马莉
WANG Zuolong,ZHONG Naifeng,MA Li

• 1:贵阳医学院附院中心实验室
• 2:贵阳医学院生物与工程学院生物技术教研室

摘要(Abstract)：

目的:研究强直性脊柱炎(AS)不同活动期辅助性T细胞1(Th1)、辅助性T细胞2(Th2)和调节性T细胞(Treg)量的改变及临床意义。方法:采用细胞内因子染色技术(ICS)及流式细胞术(FCM)检测78例AS患者(其中高活动性AS 34例、低活动性AS 44例)和30例健康体检者(正常对照组)外周血中Th1细胞、Th2细胞和Treg细胞数量,分析其与AS活动性的相关性。结果:在高活动性AS组、低活动性AS组和AS组外周血Th1细胞的量均明显高于正常对照组(P<0.01),低活动性AS组外周血Th1细胞的量明显低于高活动性AS组(P<0.01);高活动性AS组和AS组外周血Th2细胞的量均明显低于正常对照组和低活动性AS组(P<0.05);AS患者外周血Treg细胞,AS疾病组与正常对照组差异无统计学意义(P>0.05),AS患者低活动性组外周血Treg细胞与正常对照组差异无统计学意义(P>0.05),但高活动性AS组外周血Treg细胞与对照组比较明显减低,差异有统计学意义(P<0.01),AS患者高活动性组外周血Treg细胞与AS患者低活动性组比较明显减低,差异有统计学意义(P<0.01);相关性分析显示,AS患者外周血Th1细胞比例与Bath强直性脊柱炎病情活动指数(BASDI)呈正相关,Th2细胞和Treg细胞与BASDI均呈负相关。结论:AS患者外周血Th1、Th2、Treg细胞数量的改变,与AS的发生、发展有明显的相关性,检测AS患者外周血Th1、Th2、Treg细胞的数量有助于AS病情的判断。
Objective: To investigate the changes of T helper cells 1( Th1),T helper cells 2( Th2)and regulatory T cells( Treg) in the peripheral blood of ankylosing spondylitis( AS) patients in the different active stages of development of AS and their clinical significance. Methods: Seventy-eight cases of AS patients were selected as AS group and 30 cases as healthy group( normal control group),and AS group was divided into low activity AS group( 44 cases) and high activity AS group( 34 cases). The flow cytometry( FCM) and intracellular cytokine staining technique( ICS) were adopted to determine the number of Th1,Th2 and Treg cells in peripheral blood of all groups and analyzed the correlation between these cell number and AS activity. Results: The number of Th1 cells in high-activity AS group,low-activity AS group and AS group was signifcantly higher than that of normal control group( P < 0. 01),while the number of Th1 cells in low-activity AS group were significantly lower than that in high-activity group( P < 0. 01). The number of Th2 cells in high-activity AS group and AS group were significantly lower than that in low-activity group and control group( P < 0. 01). There was no statistical difference in the number of Treg cells between low-activity AS group,AS group and normal control group( P > 0. 05). The number of Treg cells in high-activity AS group was significantly lower than that in low-activity AS group and normal control group( P < 0. 01). Correlation analysis showed the percentage of Th1 cells was positively correlated to bath ankylosing spondylitis disease activity index( BASDI) while the percentage of Th2 and Treg cells was negatively correlated to BASDI.Conclusions: The percentage changes of Th1 cells,Treg cells in the peripheral blood of AS patients are significantly related to occurrence and development of AS. The detection of Th1 cells,Th2 cells and Treg cells in the peripheral blood can contribute to judgement of AS disease.

关键词(KeyWords)： 脊柱炎,强直性;辅助性T细胞1;辅助性T细胞2;调节性T细胞Treg;免疫失衡
spondylitis,ankylosing;T helper cells 1;T helper cells 2;regulatory T cells;immunologic dissonance

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅社会发展攻关项目(黔科合LG字[2011]034号)

作者(Author): 王作龙,钟乃风,马莉
WANG Zuolong,ZHONG Naifeng,MA Li

DOI: 10.19367/j.cnki.1000-2707.2015.04.010

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