谭丹,朱迪,陆苑,王爱民,黄跃伟,李勇军,兰燕宇
TAN Dan,ZHU Di,LU Yuan,WANG Aimin,HUANG Yuewei,LI Yongjun,LAN Yanyu

• 1:贵州医科大学药学院
• 2:贵州医科大学贵州省药物制剂重点实验室
• 3:贵州医科大学民族药与中药开发应用教育部工程研究中心
• 4:贵州德良方药业股份有限公司

摘要(Abstract)：

目的:建立益肾养元颗粒中陈皮的薄层色谱鉴别与橙皮苷含量的测定方法。方法:采用薄层色谱法对陈皮的定性鉴别,采用高效液相色谱法测定橙皮苷的含量;色谱柱为迪马C18柱(150×4.6,5μm),流动相为乙腈-0.1%磷酸(20∶80),流速1 m L/min,柱温45℃,检测波长283 nm。结果:薄层色谱鉴别斑点清晰,阴性对照无干扰;橙皮苷进样量为0.114~2.29μg,与峰面积呈良好线性关系(r=0.999 4),平均回收率为99.67%,平均峰面积(RSD)为2.1%。结论:所建立的方法简便、准确、重复性好,可用于益肾养元颗粒中陈皮的鉴别和橙皮苷的含量测定。
Objective: To establish the method for the identification of Pericarpium citri reticu Latae in Yishenyangyuan granules and the hesperidin content determination. Methods: Pericarpium citri reticulatae were identified by TLC and the content of hesperidin was determined by HPLC. A Diamonsil C18column( 150 mm × 4. 6 mm,5 μm) was used with a mobile phase consisting of acetonitrile-0. 1%phosphoric acid( 20∶ 80) at a flow rate of 1. 0 m L / min,the column temperature was 45 ℃ and the detection wave length was 283 nm. Result: The bilirubin spot of TLC were clear,and there was no interference in negative control. The sample size of hesperidin was in the range of 0. 114 ~ 2. 29 μg,demonstrating a good linear relationship with the peak area( r = 0. 999 4). The average recovery rate was99. 67%,and RSD was 2. 1%. Conclusion: The above established methods were simple and accurate with good reproducible characteristic,which can be used for the identification of Pericarpium citri reticulatae and content determination of hesperidin in Yishenyangyuan granules.

关键词(KeyWords)： 色谱法,薄层;益肾养元颗粒;陈皮;色谱法,液相;橙皮苷
chromatography,thin layer;Yishenyangyuan granules;Pericarpium citri reticulatae;chromatography,liquid;hesperidin

Abstract:

Keywords:

基金项目(Foundation): 贵州省中药现代化专项项目[黔科合中药字(2013)5062号,黔科合重G字(2013)4001];; 贵州省高等学校创新能力提升计划[黔教合协同创新字(2013)04]

作者(Author): 谭丹,朱迪,陆苑,王爱民,黄跃伟,李勇军,兰燕宇
TAN Dan,ZHU Di,LU Yuan,WANG Aimin,HUANG Yuewei,LI Yongjun,LAN Yanyu

DOI: 10.19367/j.cnki.1000-2707.2015.10.009

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