胡玉红,宋美玉
HU Yuhong,SONG Meiyu

• 1:佳木斯大学附属第一医院妇产科
• 2:佳木斯大学临床医学院

摘要(Abstract)：

目的:研究巨噬细胞与子宫内膜异位症(EMT)发生之间的关系。方法:选取卵巢巧克力囊肿和子宫肌瘤患者各20例,在腹腔镜下进行手术治疗,术前分别抽取约15 m L的腹腔液,制成单细胞悬液,运用显微成像技术、Cell-sense standard系统、Image J系统测量两组患者腹腔液中巨噬细胞的运动速度以及子宫内膜细胞4 h时面积的变化,并用流式细胞仪检测腹腔液中表达特异性标记物的巨噬细胞含量;比较两组患者腹腔积液中CD14+巨噬细胞不同标记物所占百分比差异。结果:实验组巨噬细胞移动速度小于对照组患者巨噬细胞移动速度,差异有统计学意义(P<0.05);实验组子宫内膜细胞面积增长,对照组患者面积减小,两组比较差异有统计学意义(Z=-4.599,P<0.05);实验组患者腹腔液中表达CD69、CD71和CD54的巨噬细胞占CD14+巨噬细胞的百分比明显低于对照组(P<0.05),差异有统计学意义。结论:巨噬细胞功能的降低可能会引起逆流内膜组织在腹腔中形成新的病灶,导致EMT的发生。
Objective: To study the relationship between macrophages and endometriosis. Methods:Twenty patients with ovarian chocolate cysts and 20 patients with uterine fibroids were selected; all patients underwent laparoscopic surgery. Approximately 15 m L peritoneal fluid was extracted before operation and single cell suspension was made. The microscopic imaging technique,Cell-sense standard system and Image J system were used to examine the trajectory,velocity of macrophages,as well as the changes in areas of endometrial cells within 4 hours. The ratio of macrophages expressed specific immunological markers measured by the flow cytometry was also calculated. The indicators mentioned above were collected and compared between the two groups. Results: The rate of macrophage movement of experimental group was lower than that in control group( P < 0. 05); the growth of endometrial cell areas of in experimental group was significant higher than that in control group( Z =- 4. 599,P <0. 05); and the ratio of macrophages expressed specific immunological markers of CD69,CD71 and CD54 was statistically lower than that in control group( P < 0. 05). Conclusion: The decrease of macrophage function may cause a backflow of endometrial tissue to form new lesions in the abdominal cavity,causing EMT.

关键词(KeyWords)： 巨噬细胞;子宫内膜异位症;移动速度
macrophage;endometriosis;movement speed

Abstract:

Keywords:

基金项目(Foundation): 佳木斯大学重点科技项目资助,项目编号(Sz2014-008)

作者(Author): 胡玉红,宋美玉
HU Yuhong,SONG Meiyu

DOI: 10.19367/j.cnki.1000-2707.2016.04.015

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