贵州医科大学学报

2019, v.44;No.229(10) 1179-1183

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Nrf2在脓毒症合并急性肾损伤大鼠肾组织中的表达及姜黄素对其的干预作用
Expression of Nrf2 in Renal Tissues of Sepsis Rats and Intervention of Curcumin

杨挺;俞慧;黄伟;
YANG Ting;YU Hui;HUANG Wei;Department of Emergency, Affiliated Hospital of Jiangnan University (Wuxi Third People's Hospital);

摘要(Abstract):

目的:探讨核因子相关因子(Nrf2)在脓毒症合并急性肾损伤(AKI)大鼠肾组织中的表达,以及姜黄素对Nrf2的干预作用。方法:96只SD大鼠随机分为对照组、模型组及治疗组,模型组和治疗组用盲肠结扎穿刺法制备脓毒症AKI大鼠模型,治疗组在制模后1 h给予100 mg/kg剂量姜黄素腹腔注射;于造模后6、12、24及48 h时,检测各组大鼠血清尿素氮(BUN)及肌酐(Scr)水平,同时取肾脏组织、采用Wester blot法检测肾脏组织匀浆中Nrf2蛋白表达水平,采用苏木精-伊红(HE)染色观察造模12 h时3组大鼠肾脏组织学变化。结果:造模后6、12、24及48 h时,与对照组同时点比较,模型组大鼠血清BUN及Scr水平显著升高,大鼠肾脏组织匀浆中Nrf2蛋白水平显著降低,差异有统计学意义(P<0.01);与模型组同时点比较,治疗组大鼠血清BUN及Scr水平显著降低,大鼠肾脏组织匀浆中Nrf2蛋白水平显著升高,差异有统计学意义(P<0.01);HE染色结果可见,模型组大鼠肾脏组织表现为炎症细胞浸润,肾小球周围间质充血,治疗组大鼠肾脏组织的这些改变则均有明显改善。结论:姜黄素可改善脓毒症AKI肾脏功能,其机制可能与上调大鼠肾脏组织中Nrf2蛋白表达水平有关。
Objective: To explore the possible mechanism of nuclear factor-related factor(Nrf2) in the occurrence and development of sepsis and its role in septic acute kidney injury(AKI), and the intervention of curcumin on Nrf2, so as to provide theoretical basis for clinical prevention and treatment of septic AKI. Methods: 96 SD rats were randomly divided into 3 groups: the control group, the operation group and the treatment group. They were further divided into 4 sub-groups in 6, 12, 24 and 48 h after operation. Sepsis AKI rat model was established by cecum ligation and puncture in the operation and treatment groups. Curcumin was injected into abdominal cavity at a dose of 100 mg/kg 1 hour after the model was established in the treatment group. The expression of Nrf2 protein in renal tissues of each group was detected by immunoblotting. Blood was collected from the heart to measure serum urea nitrogen(BUN) and creatinine(Scr). Results: Nrf2 expression was found in renal tissues of the control group. Nrf2 in the operation group increased gradually from the 6~(th) h and decreased 12 h later after modeling, significantly lower than that of the control group(P<0.05). The expression of Nrf2 in the treatment group increased gradually from the 6~(th) h, and decreased 24 h later, significantly higher than that in the operation group(P<0.05). BUN and Scr values of the operation group were significantly higher than those in the control group(P<0.05), while they were significantly lower in the treatment group than in the operation group(P<0.05). Conclusion: Sepsis infection attack destroys the endogenous protective system and inhibits the antioxidant stress response and immune response of the body, leading to AKI. Curcumin can increase the expression of Nrf2 in septic kidney tissues, improve the antioxidant capacity of kidney tissues, and has certain protective ability against AKI.

关键词(KeyWords): 姜黄素;脓毒症;核因子相关因子;氧化应激;模型,动物
curcumin;sepsis;nuclear factor-related factor;oxidative stress;model,animal

Abstract:

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基金项目(Foundation): 无锡市卫生局科研项目(MS201415)

作者(Author): 杨挺;俞慧;黄伟;
YANG Ting;YU Hui;HUANG Wei;Department of Emergency, Affiliated Hospital of Jiangnan University (Wuxi Third People's Hospital);

Email:

DOI: 10.19367/j.cnki.1000-2707.2019.10.012

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