茅向军,鲍家科,许乾丽,左鼎,王德甫
MAO Xiangjun,BAO Jiake,XU Qianli,ZUO Ding,WANG Defu(Guizhou Provincial Institute for Drug Control

• 1:贵州省药品检验所

摘要(Abstract)：

目的:建立用高效液相色谱法测定杠板归中绿原酸的含量的方法。方法:采用高效液相色谱法,Hypersil ODS2 C18柱,流动相为乙腈-0.4%磷酸(10∶90),检测波长为327 nm。结果:绿原酸在0.042～1.14μg范围内线性关系良好,平均回收率为99.19%,RSD为1.7%。结论:本分析方法简便、准确,可作为该药材质量控制的定量分析方法。
Objective: To establish a method for determinating chlorogenic acid in Polygonum perfoliatum L.with high performance liquid chromatography(HPLC).Methods: The HPLC method was carried out on C18(250 mm×4.6 mm,5 μm)column using acetonitrile-0.4% phosphoric acid(10∶90,v∶v) as mobile phase,and the flow rate was 1.0 ml · min-1.Detection wavelength was 327 nm.Result: The calibration curve for chlorogenic acid was linear in the range of 0.042～1.14 μg.The average recovery was 99.19%(n=6),and PSD was 1.7%.Conclusion: The method is convenient and accurate for quantitative determination of chlorogenic acid in P.perfoliatum L.

关键词(KeyWords)： 杠板归;绿原酸;色谱法,高压液相
Polygonum perfoltiarum;chlorogenic acid;chromatography,high pressure liquid

Abstract:

Keywords:

基金项目(Foundation): 贵州省中药现代化科技产业研究开发专项[黔科合社字(2008)5002号]

作者(Author): 茅向军,鲍家科,许乾丽,左鼎,王德甫
MAO Xiangjun,BAO Jiake,XU Qianli,ZUO Ding,WANG Defu(Guizhou Provincial Institute for Drug Control

DOI: 10.19367/j.cnki.1000-2707.2010.06.007

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