贵州医科大学学报

2020, v.45;No.236(05) 616-620

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液基薄层细胞制片技术联合细胞DNA定量分析对良恶性胸腹水患者的诊断价值
Diagnostic Value of Liquid Based Thin Layer Cell Sectioning Technique Combined with Quantitative Analysis of Cellular DNA in Pleural and Abdominal Ascites Derived from Patients with Benign and Malignant

李楣;张丽丽;姚蓓;夏静;余文静;肖娟;候秋萍;
LI Mei;ZHANG Lili;YAO Bei;XIA Jing;YU Wenjing;XIAO Juan;HOU Qiuping;Department of Clinical Laboratory,the Thirteenth People's Hospital of Chongqing;

摘要(Abstract):

目的:探究液基薄层细胞制片技术联合细胞DNA定量分析对良恶性胸腹水患者的诊断价值。方法:收集97例疑似肿瘤患者的胸腹水标本,以组织病理学诊断为标准,采用液基薄层细胞制片技术检查胸腹水中可疑癌细胞或癌细胞,采用福尔根染色法对胸腹水细胞DNA进行定量、计算DNA指数(DI)及DNA含量,分析上述2种方法单独检测及联合检测对良恶性病变的诊断符合率、灵敏度、特异度、阳性预测值及阴性预测值。结果:液基薄层细胞制片、细胞DNA定量分析分别与组织病理学诊断阳性率相比,差异均有统计学意义(P <0. 05); 2种方法联合检测的诊断符合率、诊断灵敏度和阴性预测值均分别高于液基薄层细胞制片和细胞DNA定量分析(P <0. 05)。结论:液基薄层细胞制片技术联合细胞DNA定量分析胸腹水良恶性性质具有较高的诊断符合率、灵敏度和阴性预测值。
Objective: To explore the diagnostic value of liquid-based thin layer cell sectioning technology combined with quantitative analysis of cellular DNA in patients with benign and malignant pleural and ascites. Methods: We collected the pleural and abdominal ascites specimens from 97 patients with suspected tumors based on histopathological diagnosis. Liquid-based thin-layer cell preparation technique was used to examine suspicious cancer cells or cancer cells in the pleural and ascites. DNA was quantified in cells in the pleural and ascites using Forgen staining. DNA index( DI)and DNA content were calculated. We compared the effect of the above two methods alone and their combination on diagnostic accuracy,sensitivity,specificity,positive predictive values and negative predictive values of detecting benign and malignant lesions. Results: Both liquid-based thin-layer cell preparation and quantitative analysis of cellular DNA were statistically different from the positive rate of histopathological diagnosis( P < 0. 05). The diagnostic coincidence rate,diagnostic sensitivity and the negative predictive values were higher in combination of two methods than those in liquid-based thin layer cell preparation or quantitative analysis of cellular DNA( P < 0. 05). Conclusion: Liquid-based thinlayer cell preparation technology combined with quantitative analysis of cell DNA for benign and malignant properties of pleural and ascites fluid has a high diagnostic coincidence rate,sensitivity and negative predictive value.

关键词(KeyWords): 肿瘤;胸水;腹水;诊断;液基薄层细胞制片;DNA定量分析
neoplasms;hydrothorax;ascites;diagnosis;liquid-based thin layer cell sectioning;DNA quantitative analysis

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基金项目(Foundation): 重庆市科卫联合医学科研项目(2018MSXM120)

作者(Author): 李楣;张丽丽;姚蓓;夏静;余文静;肖娟;候秋萍;
LI Mei;ZHANG Lili;YAO Bei;XIA Jing;YU Wenjing;XIAO Juan;HOU Qiuping;Department of Clinical Laboratory,the Thirteenth People's Hospital of Chongqing;

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DOI: 10.19367/j.cnki.2096-8388.2020.05.021

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