胡赟;梁文妹;
HU Yun,LIANG Wenmei(Department of Histology and Embryology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

• 1:贵阳医学院组织学与胚胎学教研室

摘要(Abstract)：

目的:研究生长抑素(somatostatin,SS)在海洛因依赖大鼠颌下腺的表达。方法:正常SD大鼠,随机分为正常对照组、盐水对照组及海洛因依赖组,皮下注射海洛因建立海洛因依赖大鼠模型,分别于第10、17、24、31、38天取颌下腺,应用免疫组织化学SABC法及图像分析方法检测SS的表达。结果:SS阳性细胞数量及免疫反应强度在海洛因依赖第10天下降,之后随成瘾时间延长呈逐渐增强趋势;图像分析显示,SS阳性细胞数量在第31天时最高(P<0.05),平均灰度值在第31天时最低(P<0.05)。结论:海洛因依赖期间,生长抑素在大鼠颌下腺表达水平的变化可能是机体对海洛因依赖产生的一种适应性调节过程。
Objective:To observe the expression of somatostatin (SS) in submandibular gland of heroin dependent rats. Methods: Seventy SD rats were divided into normal control group (NCG,n=10),saline control group (SCG,n=30) and heroin dependence group (HDG,n=30). Heroin dependence model of rats were established by injecting with heroin subcutaneously. The immunohistochemical SABC method and image analysis were used to detect SS expression in submandibular gland on the 10th,17th,24th,31st and 38th days after the injection respectively. Results: The number of SS positive cells and staining intensity in submandibular gland decreased obviously in HDG,on the 10th day and then increased gradually along with the prolongation of heroin dependent time. The peak of SS positive cell number was on the 31st day showed by image analysis (P<0.05),while the lowest value (P<0.05) of mean grey degree was obtained on the same day. Conclusions: The changes of SS expression in rat submandibular gland during heroin dependence might be an adjusting process of the organism to injury caused by heroin.

关键词(KeyWords)： 海洛因依赖;生长抑素;颌下腺;免疫组织化学
heroin dependence;somatostatin;submandibular gland;immunohistochemistry;rats,sprague-dawley

Abstract:

Keywords:

基金项目(Foundation): 贵州省优秀科技教育人才省长专项资金资助项目:黔科教办[2001-3];; 教育部“春晖计划”项目:Z2005-1-52020

作者(Author): 胡赟;梁文妹;
HU Yun,LIANG Wenmei(Department of Histology and Embryology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

Email:

DOI: 10.19367/j.cnki.1000-2707.2009.05.003

参考文献(References)：

• [1]Osterbrink J,Haas U.Opioid-induced bowel dysfunc-tion:a literature analysis on pathophysiology and treat-ment[J].Wien Med Wochenschr,2008(21-22):621-626.
• [2]Holzer P.Treatment of opioid-induced gut dysfunction.Expert Opin Investig Drugs,2007(2):181-194.
• [3]耿嘉蔚,郭强,马德林,等.海洛因成瘾者戒毒后部分胃肠肽类激素的测定[J].诊断学理论与实践,2003(2):138-139.
• [4]Elitok B,Elitok OM,Ketani MA,et al.The effect of dex-amethasone on gastric mucosal changes following sialoade-nectomy in rat[KJ].J Endocrinol Invest,2005(8):700-703.
• [5]Sabbatini ME.Natriuretic peptides as regulatory mediatorsof secretory activity in the digestive system[J].Regul Pept,2009(1-3):5-15.
• [6]Stumpf WE,Hayakawa N.Salivary glands epithelial and myoepithelial cells are major vitamin D targets[J].Eur J Drug Metab Pharmacokinet,2007(3):123-129.
• [7]Pawlikowski M,Melen-Mucha G.Perspectives of new potential therapeutic applications of somatostatin analogs[J].Neuro Endocrinol Lett,2003(1-2):21-27.
• [8]Hatzoglou A,Kampa M,Castanas E.Opioid-somatosta-tin interactions in regulating cancer cell growth[J].Front Biosci,2005(10):244-256.
• [9]Meerpohl JJ,Timmer A.News from the cochrane library:mu opioid antagonists for opioid-induced bowel dysfunc-tion[J]Z Gastroenterol,2008(9):917-921.
• [10]高峻钰,刘少君.新合成的生长抑素SOMa对吗啡依赖大鼠的影响[J].中国药物依赖性杂志,2005(1):16-19.

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