黄海,冯发莉,刘杨,冉贵萍
HUANG Hai,FENG Fali,LIU Yang,RAN Guiping(Department of Clinical Laboratory Science

• 1:贵阳医学院医学检验系临床生化教研室

摘要(Abstract)：

目的:用明胶海绵吸收法回收DNA片段,并验证该方法能否有效、快速的从电泳后琼脂糖凝胶中回收DNA片段。方法:将明胶海绵剪成楔形,在紫外灯照射下插入含有目的DNA片段的琼脂糖凝胶中,待海绵完全膨胀后取出,离心收集DNA溶液;并用该法回收的DNA片段进行PCR扩增,检验其回收的DNA质量。结果:明胶海绵吸收法回收DNA样品的得率介于63%～98%,所回收的DNA进行PCR扩增能够获得相应的目的条带。结论:明胶海绵吸收法是一种快速、有效的DNA回收方法。
Objective:To develop a novel method for DNA recovery,and verify its feasibility in extraction and purification of DNA segments from agarose gel after electrophoresis. Methods:A piece of gelatin sponge was cut to a wedge-form stick and inserted into agarose gel at place of goal DNA fragment under long-wavelength UV. DNA solution was collected by centrifuging after the sponge was fully swelled. The amount and purity of DNA sample were determined by PCR. Results:DNA yield rates were in the range of 63~98%,and the target DNA fragments amplified by PCR were intact. Conclusion:The sponge absorption method is feasible,effective and fast for DNA recovery.

关键词(KeyWords)： 电泳,琼脂凝胶;明胶海绵,吸收性;DNA
electrophoresis,agar gel; gelatin sponge,absorbable; DNA

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技基金资助项目[黔科合J字(2007)2085号]

作者(Author): 黄海,冯发莉,刘杨,冉贵萍
HUANG Hai,FENG Fali,LIU Yang,RAN Guiping(Department of Clinical Laboratory Science

DOI: 10.19367/j.cnki.1000-2707.2010.03.018

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