许乾丽,茅向军,熊慧林
XU Qianli,MAO Xiangjun,XIONG Huilin(Guizhou Provincial Institute for Drug Control

• 1:贵州省药品检验所

摘要(Abstract)：

目的:建立用高效液相色谱法测定六味安消胶囊中游离蒽醌、总蒽醌和结合蒽醌含量(以大黄素和大黄酚的总量计)的方法。方法:采用依利特C18柱,流动相为甲醇-0.1%磷酸溶液(80∶20),检测波长为254 nm。结果:大黄素、大黄酚分别在0.016 2～0.323 2μg和0.035 6～0.712 8μg范围内线性关系良好,游离蒽醌中大黄素和大黄酚的平均回收率分别为100.08%、99.11%,RSD分别为0.37%、2.70%;总蒽醌中大黄素和大黄酚的平均回收率分别为101.62%、100.54%,RSD分别为2.40%、1.37%。结论:HPLC法简便,准确,可作为六味安消胶囊的质量控制定量分析方法。
Objective: To develop an HPLC method for determination of free anthraquinone,total anthraquinone and binding anthraquinone in Liuwei Anxiao capsules.Methods: Hypersil C18 column(200 mm×5.0 mm,5 μm) was adopted.The mobile phase was methnol-0.1% phosphoric acid(80∶20).The flow rate was 1 mL·min-1 and the detection wavelength was 254 nm.Results: The linear ranges of emodin and chrysophanol were 0.016 2～0.323 2μg and 0.0356～0.7128μg,respectively.The average recoveries were 100.08%(RSD=0.37%) for emodin,and 99.11%(RSD=2.70%) for chrysophanol in free anthraquinone,and 101.62%(RSD=2.40%) for emodin,and 100.54%(RSD=1.37%) for chrysophanol in total anthraquinone,respectively.Conclusion: The method is convenient,accurate and can be used for determination of the preparation.

关键词(KeyWords)： 色谱法,高压液相;蒽醌类;大黄素;大黄酚
chromatrography,high pressure liquid;anthraquinones;emodin;chrysophanol

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 许乾丽,茅向军,熊慧林
XU Qianli,MAO Xiangjun,XIONG Huilin(Guizhou Provincial Institute for Drug Control

DOI: 10.19367/j.cnki.1000-2707.2010.06.012

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