鲍玲娜,马洪,李超,孙俊泽
BAO Lingna,MA Hong,LI Chao,SUN Junze

• 1:贵州医科大学附属口腔医院口腔颌面外科
• 2:贵州医科大学
• 3:四川省肿瘤医院.研究所四川省癌症防治中心头颈外科

摘要(Abstract)：

目的:探讨外源性S100A8/A9蛋白对人舌鳞癌细胞SCC-25及CAL-27侵袭能力的影响。方法:人舌鳞癌SCC-25及CAL-27细胞分别分为实验组及对照组,实验组用5 mg/L S100A8/A9蛋白干预24 h,对照组添加等量培养基;采用Transwell小室侵袭实验检测SCC-25及CAL-27细胞侵袭情况,实时定量PCR(RT-qPCR)检测SCC-25及CAL-27细胞中基质金属蛋白酶-2(MMP-2)及MMP-9基因的表达情况。结果:与对照组比较,实验组SCC-25及CAL-27细胞在Transwell小室中降解人工基底膜并穿过聚碳酸酯膜的能力增强(P<0.05),且实验组SCC-25及CAL-27细胞MMP-2及MMP-9的基因相对表达量增加(P<0.05)。结论:5 mg/L外源性S100A8/A9蛋白能增强舌癌SCC-25及CAL-27细胞的侵袭能力,其机制可能与S100A8/A9蛋白影响两种细胞中MMP-2及MMP-9表达有关。
Objective: To investigate the effect of exogenous S100 A8/A9 protein on the invasive ability of human tongue squamous cell carcinoma(TSCC) SCC-25 and CAL-27 and to provide a theoretical basis for the study of the mechanism of invasion and metastasis of S100 A8/A9 protein in tongue squamous cell carcinoma. Methods: Human tongue squamous cell carcinoma scc-25 and cal-27 cells were divided into the experimental group and the control group respectively. The experimental group was treated with 5 mg/L S100 A8/A9 protein for 24 h, and the control group was treated with the same amount of medium.Transwell cell invasion assay was used to detect the invasion of S100 A8/A9 protein on SCC-25 and CAL-27 cells after 24 hours. RT-q PCR was used to detect the expression of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9) genes in SCC-25 and CAL-27 cells. Results: Compared with the control group, adding 5 mg/L exogenous S100 A8/A9 protein could enhance the ability of SCC-25 and CAL-27 cells of the experimental group to degrade artificial basement membrane and pass through polycarbonate membrane in Transwell chamber(P<0.05),and the relative expression of MMP-2 and MMP-9 genes was increased(P<0.05). Conclusion: 5 mg/L exogenous S100 A8/A9 protein can enhance the invasion ability of tongue cancer scc-25 and cal-27 cells, and the mechanism may be related to the influence of S100 A8/A9 protein on the expression of mmp-2 and mmp-9,which may provide a potential biomarker for targeted therapy of tongue squamous cell carcinoma.

关键词(KeyWords)： 舌肿瘤;癌;钙卫蛋白;细胞侵袭;基质金属蛋白酶-2;基质金属蛋白酶-9
tongue neoplasms;carcinoma;calprotectin;cell invasion;matrix metalloproteinases-2;matrix metalloproteinases-9

Abstract:

Keywords:

基金项目(Foundation): 贵州省教育厅项目[黔科合外KY字(2017)005]

作者(Author): 鲍玲娜,马洪,李超,孙俊泽
BAO Lingna,MA Hong,LI Chao,SUN Junze

DOI: 10.19367/j.cnki.1000-2707.2019.11.008

参考文献(References)：

• [1] SIEGEL R L,MILLER K D,JEMAL A.Cancer statistics,2018[J].CA Cancer J Clin,2018,68(1):7-30.
• [2] BRAY F,FERLAY J,SOERJOMATARAM I,et al.Global cancer statistics 2018;GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J].CA Cancer J Clin,2018,68(6):394-424.
• [3] BRESNICK A R,WEBER D J,ZIMMER D B.S100 proteins in cancer[J].Nat Rev Cancer,2015,15:96-100.
• [4] SHABANI F,FARASAT A,MAHDAVI M,et al.Calprotectin (S100A8/S100A9):A key protein between inflammation and cancer[J].Inflamm Res,2018,67(10):801-812.
• [5] ANG C W,NEDJADI T,SHEIKH A A,et al.Smad4 loss is associated with fewer S100A8-positive monocytes in colorectal tumors and attenuated response to S100A8 in colorectal and pancreatic cancer cells[J].Carcinogenesis,2010,31(9):1541-1551.
• [6] KWON C H,MOON H J,PARK H J,et al.S100A8 and S100A9 promotes invasion and migration through p38 mitogen-activated proteinkinase-dependent NF-κB activation in gastric cancer cells[J].Mol Cells,2013,35(3):226-234.
• [7] 何芹.钙结合蛋白S100A8和S100A9对鼻咽癌侵袭和迁移的影响[D].南宁:广西医科大学,2015.
• [8] CHEN H,XU C,JIN Q,et al.S 100 protein family in human cancer[J].Am J Cancer Res,2014,4(2):89-115.
• [9] HOMA-MLAK I,MAJDAN A,MLAK R,et al.Metastatic potential of NET in neoplastic disease[J].Postepy Hig Med Dosw,2016,70(10):887-895.
• [10]GHAVAMI S,RASHEDI I,DATTILO B M,et al.S100A8/A9 at low concentration promotes tumor cell growth Via RAGE ligation and MAP kinase-dependent pathway[J].Leukoc Biol,2008,83(6):1484-1492.
• [11]SCHIOPU A,COTOI OS.S100A8 and S100A9:DAMPs at the crossroads between innate immunity,traditional risk factors,and cardiovascular disease[J].Mediators Inflamm,2013,2013:828354.
• [12]GOYETT E J,GECZY C L.Inflammation-associated S100 proteins:new mechanisms that regulate function[J].Amino Acids,2011,41(4):821-842.
• [13]SINHA P,OKORO C,FOELL D,et al.Proinflammatory S100 proteins regulate the accumulation of myeloid-derived suppressor cells[J].J Immunol,2008,181(7):4666-4675.
• [14]GABRILOVICH D I.Myeloid-derived suppressor cells[J].Cancer Immunol Res,2017,5(1):3-8.
• [15]HANAHAN D,WEINBERG R A.The hallmarks of cancer[J].Cell,2000,100:57-70.
• [16]RADISKY E S,RADISKY D C.Matrix metalloproteinases as breast cancer drivers and therapeutic targets[J].Front Biosci,2015,20:1144-1163.
• [17]JAFARIAN A H,VAZIFE MOSTAAN L,MOHAMMADIAN ROSHAN N,et al.Relationship between the expression of matrix metalloproteinase and clinicopathologic features in oral squamous cell carcinoma[J].Iran J Otorhinolaryngol,2015,27(80):219-223.

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