陆苑,孙佳,陈思颖,郑林,郑鲁平,兰燕宇
LU Yuan,SUN Jia,CHEN Siying,ZHENG Lin,ZHENG Luping,LAN Yanyu

• 1:贵州医科大学药学院
• 2:贵州省药物制剂重点实验室
• 3:民族药与中药开发应用教育部工程研究中心
• 4:贵州华南理工生物工程有限公司

摘要(Abstract)：

目的:观察梨麦消食咀嚼片对大鼠和小鼠胃肠道消化功能的促进作用。方法:50只小鼠设置空白对照组、便秘模型对照组和受试物(梨麦消食咀嚼片)低、中、高剂量组,另取40只SD大鼠设置空白对照组和受试物低、中、高剂量组,所有受试物组动物分别给予0.32、0.65及1.94 g/kg梨麦消食咀嚼片连续灌胃31 d,空白对照组和模型对照组给予等量生理盐水灌胃;便秘模型组和受试物组小鼠用复方地芬诺酯制作便秘模型,各组小鼠进行小肠运动实验,计算墨汁推进率;记录各组大鼠实验前及灌胃后第7、14、21及30天时的体质量,观察各组大鼠灌胃31 d时的总摄食量、食物利用率、胃蛋白酶活性和胃蛋白酶排出量。结果:中、高剂量组小鼠的墨汁推进率显著高于便秘模型对照组(P<0.05);与空白对照组比较,高剂量的梨麦消食咀嚼片能提高大鼠的胃液量和胃蛋白酶活性(P<0.05),中、高剂量的梨麦消食咀嚼片能增加大鼠胃蛋白酶排出量(P<0.05),3种剂量的梨麦消食咀嚼片对大鼠体质量增重、摄食量及食物利用率无明显影响(P>0.05)。结论:梨麦消食咀嚼片有促进大鼠和小鼠消化功能的作用。
Objective: To observe the promoting role of"Li Mai Xiao Shi(LMXS) chewable tablet"on digestive function of rats and mice. Methods: 50 mice were divided into blank control group,constipation model group and experimental test group(LMXS chewable tablets),which was further to divided into low,middle and high dose group. Meanwhile,40 SD rats were divided into blank control group,and experimental test group(low,middle and high dose group). All the experimental test groups were given intragastric administration of 0. 32,0. 65 and 1. 94 g/kg animal LMXS chewable tablets for 31 days,while blank control group and constipation model group were given intragastric administration of normal saline. The constipation model was established with compound diphenoxylate in the constipation model group and the experimental test group of mice,and the small intestine movement experiment was adopted to calculate ink advance rate. The body weight of rats in each group before intragastric administration and at 7,14,21 and 30 days after intragastric administration was recorded,and the total food intake,food utilization rate,pepsin activity and pepsin output were observed after the rats were given intragastric administration for 31 days. Result: The ink advance rate of high dose group was significantly higher than that of constipation model group(P < 0. 05). Compared with the blank control group,high dose of LMXS chewable tablets can improve the volume of gastric juice and pepsin activity of rats(P < 0. 05),and high dose of LMXS chewable tablets can increase rat pepsin excretion(P < 0. 05). The high,middle and low doses of LMXS chewable tablets had no effect on rat body weight gain,food intake and food utilization rate(P < 0. 05). Conclusion: LMXS chewable tablet can promote digestive function of rats and mice.

关键词(KeyWords)： 梨麦消食咀嚼片;小肠运动;胃蛋白酶;消化功能;大鼠,Sprague Dawley
Li Mai Xiao Shi chewable tablet;intestine movement;pepsin;digestion function;rats,Sprague-Dawley

Abstract:

Keywords:

基金项目(Foundation): 贵州省中药现代化[黔科合中药字(2011)25031];黔教合协同创新字(2013)04;黔科合平台人才(2016)561315677

作者(Author): 陆苑,孙佳,陈思颖,郑林,郑鲁平,兰燕宇
LU Yuan,SUN Jia,CHEN Siying,ZHENG Lin,ZHENG Luping,LAN Yanyu

DOI: 10.19367/j.cnki.1000-2707.2017.05.012

参考文献(References)：

• [1]高慧艳,张聪恪,陈东方,等.香术砂片促消化功能的临床研究[J].中成药,2015(37):2142-2145.
• [2]郑丹,夏冰,施先艳,等.多潘立酮联合复方消化酶治疗功能性消化不良84例[J].世界华人消化杂志,2009(3):336-339.
• [3]陈莉,吉莉.山楂麦芽片促进消化功能的动物试验研究[J].海峡药学,2015(10):13-15.
• [4]李婧,孙建栋,陈乃宏.中药姜黄中主要活性成分姜黄素的抗抑郁作用研究进展[C]//第二届中国药理学会补益药药理专业委员会学术研讨会,2012.
• [5]中华人民共和国药典[S].北京:中国医药科技出版社,2015.
• [6]卫生部卫生法制与监督司.保健食品检验与评价技术规范(2003版)[S].北京:人民卫生出版社,2003.
• [7]李俊松,赖筱娟,王华富,等.小儿消食颗粒促消化研究[J].中国实验方剂学杂志,2010(18):132-134.
• [8]王叔桥,张丽宏,刘莉,等.健胃喜食宝片促进消化功能动物试验研究[J].四川医学,2012(1):28-30.
• [9]王睿,刘丽达,蒋中仁,等.健中消食片促进消化功能动物试验研究[J].预防医学情报杂志,2014(6):436-438.
• [10]温靖,徐玉娟,肖更生,等.青梅提取物的制备及体内促消化功能研究[J].食品科学,2010(5):266-269.

文章评论(Comment)：