杨水秀,国果
YANG Shuixiu,GUO Guo

• 1:贵州医科大学基础医学院
• 2:贵州医科大学
• 3:贵阳市公共卫生救治中心血库室

摘要(Abstract)：

目的:探讨血清干扰素-γ(IFN-γ)、白细胞介素-21(IL-21)、趋化因子CXCR受体5(CXCR5)水平及血液CD8~+T淋巴细胞数与HIV感染病程进展的相关性。方法:HIV抗体确诊阳性病人75例为研究组,根据血液CD4~+T淋巴细胞计数分为A组(CD4~+T<200个/μL)、B组(CD4~+T 200～500个/μL)及C组(CD4~+T>500个/μL),另选取同期健康体检者25例为对照组;研究组于抗反转录病毒治疗(ART)前1天、ART后3个月时、健康对照组体检时采取空腹静脉血,采用流式细胞术检测CD4~+T、CD8~+T淋巴细胞计数,采用酶联免疫吸附法(ELISA)法检测血清IFN-γ、IL-21及CXCR5水平;采用Pearson相关分析HIV感染者各组所测指标与该疾病病程的相关性。结果:ART前IFN-γ、CXCR5及IL-21的水平比较,随着CD4~+T淋巴细胞计数逐渐降低,所测指标水平逐渐减少,A组0.05);各组HIV感染患者ART后血清IFN-γ、CXCR5及IL-21水平均较治疗前升高,差异有统计学意义(P<0.01);Pearson相关分析结果显示,HIV感染患者血清IFN-γ、CXCR5、IL-21水平与CD4~+T淋巴细胞、CD8~+T淋巴细胞计数呈正相关;IFN-γ、CXCR-5、IL-21水平与艾滋病病毒载量呈负相关,差异有统计学意义(P<0.05)。结论:HIV感染患者血清IFN-γ、CXCR5、IL-21水平与其疾病进展存在一定的相关性。
Objective: To investigate the changes of blood IFN-γ, CXCR5,IL-21 and CD8~+T-lymphocyte counts of HIV infected patients and their correlation with disease progression. Methods: 75 cases with positive HIV antibody were selected as the study group and divided into three groups according to the detection of CD4~+T-lymphocyte before treatment: Group A(CD4~+T<200 μL), Group B(CD4~+T 200～500 μL) and Group C(CD4~+T>500 μL). 25 healthy people were selected as the control group. Peripheral blood was detected for the study group 1 day before effective antiretroviral therapy(ART) and in 3 months after treatment and for the control group in the physical examination. Flow cytometry was used to count CD4~+T,CD8~+T-lymphocytes, and PCR was used to quantify HIV RNA. The levels of IFN-γ, CXCR5 and IL-21 in serum were detected by ELISA.The correlation between the measured indexes and progression of disease was analyzed by linear correlation. Results: The levels of IFN-γ、CXCR5、IL-21 gradually decreased with the decrease of immune function before ART, namely, Group A

关键词(KeyWords)： γ-干扰素;细胞白介素-21;趋化因子CXCR受体5;CD8~+T淋巴细胞;抗反转录病毒治疗;HIV感染
interferon-γ;interluekin-21;chemokine CXCR receptor 5;CD8~+T-lymphocytes;antiretroviral therapy;HIV infection

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81760647)

作者(Author): 杨水秀,国果
YANG Shuixiu,GUO Guo

DOI: 10.19367/j.cnki.1000-2707.2019.10.014

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