毕晓洁,李兴,杨国珍,潘卫
BI Xiaojie,LI Xing,YANG Guozhen,PAN Wei(Department of Laboratory Medicine

• 1:贵阳医学院医学检验系

摘要(Abstract)：

目的:探讨提高实验大鼠受孕率的方法及准确判断大鼠受孕的检测方法。方法:24只SD雌鼠随机分为性周期筛选合笼组(筛选组)和延长时间合笼组(延长时间组),筛选组雌鼠根据阴道涂片于发情前期与雄鼠合笼2 d,延长时间组雌鼠直接与雄鼠合笼10 d,采用阴道精子检查法和阴道精子合并角化上皮检查法判断合笼后雌鼠是否受孕,以分娩作为受孕成功的标准,观察两组受孕率,并比较两种判断受孕的方法。结果:筛选组受孕率(91.67%)高于延长时间合笼组(50.00%),差异具有统计学意义(P<0.05);延长时间组第1～5天受孕率(41.67%)高于第6～10天(14.28%),差异不具有统计学意义(P>0.05);阴道精子检查法和阴道精子合并角化上皮检查法的假阴性率分别为0和11.76%,假阳性率分别为14.29%和0。结论:性周期筛选合笼法对雌鼠受孕率高于延长时间合笼法,阴道涂片法判断雌鼠受孕需结合雌鼠性周期推算。
Objective: To investigate the method for increasing pregnant rate of rats,and to explore the methods for judging pregnant rats.Methods: Twenty-four female rats were randomly divided into 2 groups: screening sexual cycle for copulating group(screening group) and prolonging time for copulating group(prolonging time group).Rats in screening group copulated with male rats for two days in proestrus stage and their sexual cycle was detected with vaginal smear.Rats in prolonging time group copulated with male rats for 10 days directly.Whether female rats were pregnant was judged by 2 methods: vaginal sperm examination method and vaginal sperm combined with cuticular epithelium examination method.Delivery served as judging pregnancy standards.Pregnant rates of rats in screening group and in prolonging time group in the 1st to 5th days and in the 6th to 10th days were observed.Two judgmental methods were compared.Results: There was significant difference in rat pregnant rates(P<0.05)between screening group(91.67%) and prolonging time group(50.00%).The pregnant rates in prolonging time group in 1~5 days(41.67%) were higher than 6~10 days(14.28%),but the difference was not obvious(P>0.05).The false negative rates of vaginal sperm examination method and vaginal sperm combined with cuticular epithelium examination group were 0 and 11.76%,and the false positive rates of them were 14.29% and 0.Conclusions: Screening sexual cycle with vaginal smear method in improving pregnant rate is better than prolonging time method.Vaginal smear combined with screening sexual cycle helps to judging pregnant rats accurately.

关键词(KeyWords)： 阴道涂片;受孕率;判断方法;大鼠
vaginal smear;pregnant rate;judgmental method;rats

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术厅科研基金资助项目[黔科合J字(2011)2275号];; 国家级教学团队资助项目[教高函(2009)18号]

作者(Author): 毕晓洁,李兴,杨国珍,潘卫
BI Xiaojie,LI Xing,YANG Guozhen,PAN Wei(Department of Laboratory Medicine

DOI: 10.19367/j.cnki.1000-2707.2012.06.008

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