孔祥耀,于世博,黎小梅,唐毅,倪稳,陈明翠,张金娟
KONG Xiangyao,YU Shibo,LI Xiaomei,TANG Yi,NI Wen,CHEN Mingcui,ZHANG Jinjuan

• 1:贵州医科大学化学与生物化学实验室

摘要(Abstract)：

目的:研究有柄石韦孢子叶(SPP)水提物对小鼠全层切除伤的影响。方法:对75只昆明小鼠做背中线100 mm~2的全层皮肤切除制作模型;随机均分成空白组、云南白药组和有柄石韦组,云南白药组和有柄石韦组分别给予云南白药粉末和有柄石韦提取物敷于伤口,连续给药5 d;计算造模后第1~3天,第3~7天,第7~14天和第14~18天伤口缩小比例;于造模后第3、7及18天取处死小鼠,取背部皮肤组织行HE染色,观察3组小鼠伤口皮肤肉芽组织生长情况和再上皮化程度。结果:云南白药组和有柄石韦组在第1~3天、第3~7天、第7~14天和第14~18天时段的伤口缩小比例均大于对照组(P<0.05);云南白药组在第1~3天、第3~7天和第14~18天时段的伤口缩小比例高于有柄石韦组(P<0.05);造模后第3天,云南白药组和有柄石韦组的肉芽组织较对照组组织规则厚实;第7天时,云南白药组和有柄石韦组的肉芽组织厚度增加,较为紧凑,无水肿现象,成纤维细胞数量明显增高;第18天时,3组小鼠组伤口基本愈合,但云南白药组和有柄石韦组的伤口愈合后组织较对照组紧致,无水肿现象。结论:有柄石韦能促进小鼠伤口愈合,作用稍弱于云南白药。
Objective: To observe the effect of water extract of Pyrrosia petiolosa on mice's full-thickness excision wound. Methods: 75 Kunming mice received adaptive feeding for 3 days,and 100 mm~2 full thickness skin resection model was established on mice's dorsal midline. Then the mice were randomly divided into blank control group,Yunnan baiyao group and Pyrrosia petiolosa group. In Yunnan baiyao group,the powder was applied to the wound while in Pyrrosia petiolosa group the water extract of Pyrrosia petiolosa applied to the wound,and both groups received continuous administration for 5 days,once per day. Wound's area and volume reduction ratio was calculated during 1 ~ 3 days,during 3 ~ 7 days,during 7 ~ 14 days and during 14 ~ 18 days,respectively. Mice were sacrificed 3~(th),7~(th),18 ~(th)day after model establishment,HE dyeing was conducted for dorsal skin tissue. The growth of skin granulation tissue and the degree of re-epithelialization of wound skin in mice were observed in the three groups. Results: Compared with blank control group,the wound's area and volume reduction ratio in Pyrrosia petiolosa group and Yunnan Baiyao group during 1 ~ 3 days,3 ~ 7 days,7 ~ 14 days and 14 ~ 18 days was significantly higher( P < 0. 05). Compared with Pyrrosia petiolosa group,the wound's area and volume reduction ratio in Yunnan Baiyao group during 1 ~ 3 days,3 ~ 7 days,and 14~ 18 days was significantly higher( P < 0. 05). On the 7~(th)day,the thickness of granulation tissue increased,skin granulation tissue was more compact. Besides,no edema existed in skin granulation tissue and the number of fibroblasts was significantly increased. On the 18 thday,wound almost healed in all three groups,but the tissue after wound healing in Pyrrosia petiolosa group and Yunnan Baiyao group was more compact compared with blank control group. Also,there was no edema in tissue of Pyrrosia petiolosa group and Yunnan Baiyao group. Conclusion: Pyrrosia petiolosa can promote wound healing but its effect is poorer than Yunnan Baiyao.

关键词(KeyWords)： 云南白药;伤口愈合;肉芽组织;小鼠;有柄石韦
Yunnan Baiyao;wound healing;skin granulation tissue;mice;Pyrrosia petiolosa

Abstract:

Keywords:

基金项目(Foundation): 大学生创新创业基金重点项目(NO.201410660001)

作者(Author): 孔祥耀,于世博,黎小梅,唐毅,倪稳,陈明翠,张金娟
KONG Xiangyao,YU Shibo,LI Xiaomei,TANG Yi,NI Wen,CHEN Mingcui,ZHANG Jinjuan

DOI: 10.19367/j.cnki.1000-2707.2016.05.015

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